Nutritional research on the technical development for healthy seed rearing

本研究从亲鱼饲养、蛋品质改良、仔鱼消化吸收、仔鱼营养、微粒化饲料、营养价值及活体饲料强化等方面对健康鱼种苗养殖技术的发展进行了研究，获得了仔鱼种苗规模化生产的相关知识。1.试验了富含β-胡萝卜素和维生素A的卤虫无节幼体对防止仔鱼畸形和色泽异常的效果。结果表明，维生素A似乎可以有效地防止卤虫引起的异常变色，但过量的维生素C会对牙鲆的正常生长产生不良影响。2.我们认为，当食物中缺乏VA、DHA和磷脂时，眼部视网膜视紫红质的形成受到阻碍，导致黑色素的形成中断。3.单独补充维生素A、E和C对牙槽鱼卵巢发育的影响。本研究表明在亲虾日粮中添加维生素A、E和C对促进卵巢发育的重要性。4.对一种稳定的维生素C-1-2-磷酸抗坏血酸-镁(L-抗坏血酸1-2-磷酸-镁)作为维生素C来源的效果进行了试验。饲养实验结果表明，饲料中添加3 mg/100g饲料的APM足以预防斑点石斑鱼的缺乏症。5.饲料中添加EPA和DHA后，DHA仅在脑视网膜的极部积聚。

This research was conducted for the technical development of seed rearing of healthy fish : broodstock fish rearing, improvement of egg quality, digestion and absorption of fish larvae, larval nutrition, microparticulated diet, nutritional value and enrichment of live food, etc. The knowledge for seed mass production of fish larvae was obtained.1.The effect of beta-carotene and vitamin A enriched Artemia nauplii on the prevention of malformation and color abnormality of larval Japanese flounder was tested. Results suggest that VA seems to be effective in preventing the abnormal coloration caused by Artemia, but the excess amount of this vitamin can exert ill effects on normal growth of flounder.2.We suggest that the rhodopsin formation of eye retina was hindered when VA,DHA and phospholipid were deficient in foods, resulting in the interruption of black pigment formation.3.The effect of supplementation of individual vitamins A,E,and C on the ovarian development of P.iaponicus. This study showed the essentiality of supplemental vitamins A,E,and C in the diets of broodstock prawns for enhanced ovarian development4.A stable form of vitamin C, L-ascorby1-2-phosphate-Mg(APM), was tested for efficacy as a Vitamin C source in the juvenile striped jack. The results of the feeding experiment indicated that a supplement of 3mg APM/100g diet was sufficient to prevent the deficiency symptoms of the striped jack.5.When dietary EPA and DHA were fed on red sea bream, only DHA was accumulated in polar fraction of brain retina.