Pathological and Preventive Nutritional Studies on Environmental Factors of Cerebrovascular Dementia in the Japanese, from Neutritional Pathogenesis of Cerebrovascular Diseases.

我们根据合作研究者之间良好的标准化研究方案，应用新的免疫组化技术研究脑血管疾病的发病特征：脑标本取脑卒中和/或脑血管性痴呆患者死后5小时内尸检标本的穿动脉和皮质动脉（1 × 1 × 0.5cm），用Methonal-Carnoy固定液固定，石蜡包埋并切片，用于苏木精、伊红、Masson、Azan-Mallory和Elastica货车Gieson染色后的常规病理学检查以及免疫过氧化物酶染色，用抗肌动蛋白单克隆抗体和抗巨噬细胞单克隆抗体，我们证明了平滑肌细胞的变性和坏死始于外层，穿支动脉伴Virchow-Robin间隙巨噬细胞浸润和胶原增生，在晚期，巨噬细胞穿透内皮细胞并聚集在内皮下空间，以诱导闭塞性病变，如中风中的血管病变-这些发现支持了在日本脑卒中和脑血管性痴呆的脑血管疾病发病机制中紊乱的重要性。我们对日本移民的营养进行了比较流行病学调查，他们的中风发病率低于日本人。

We studied the pathogenetic feature of cerebrovascular diseases by applying new immunohistochemical techniques according to the well-standardized study protocol among collaborating investigators : Brain specimens (1*1*0.5cm)including perforating and cortical arteries from autopsied cases of stroke and/or cerebrovascular dementia were obtained within 5 hours after death were fixed in methonal-Carnoy's fixative, embedded in paraffin and sectioned for routine pathalogical examination after Hematoxilin, Eosin, Masson, Azan-Mallory and Elastica van Gieson stains as well as for immunoperoxidase staining, using monoclonal antibodies against antiactin and antimacrophage antibodies.We demonstrated the degeneration and necrosis started at the outer layr of smooth muscle cells of perforating arteries with the infiltration of macrophages from Virchow-Robin's space and collagen proliferation, and at the advanced stage macrophages penetrated through endothelial cells and accumulated in the subendothelial space to induce occlusive lesions like vascular lesions in stroke-prone SHR.These findings support the importance of disturbance in the pathogenesis of cerebrovascular diseases in stroke and cerebrovascular dementia in the Japanese.This aspect was also supported by our comparative epidemiological survey on the nutrition of Japanese immigrants who had less incidence of stroke than the Japanese in Japan.