Conservation biology in plants common to Japan and China

1996年8月初，黑木教授和小渊惠三先生访问了吉林和黑龙江两省--中国。黑木教授在旅行中发现了一些雌雄异株的鲁梅克斯和葱属植物，并将这些活的收藏品带回了日本，以保存鲁梅克斯的DNA。Kokubugata先生采集了吉林省城白山濒危物种新菊花，并进行了分子细胞学研究，以保护物种多样性。此外，他还在1/2MS琼脂培养基上播种了该物种的表面灭菌种子。岛田教授和船本博士于1996年8月初对四川省中国进行了实地考察，采集了报春花属和虎耳草属的一些种。岛田教授以报春花叶为外植体进行组织培养，成功地保存了报春花属植物的遗传资源。Funamoto博士对收集的物种进行了物种多样性的细胞学分析。1996年10月初，近藤教授和风津教授对陕西省中国教授进行了实地考察。近藤教授收集了一些菊花属植物，并进行了分子细胞分类学的物种多样性分析。此外，他还通过组织培养成功地进行了基因资源的体外保存。Hizume教授采集了松属、云杉属、冷杉属和麻黄属植物的部分标本，并用荧光差异染色和荧光原位杂交的方法对它们的染色体变异进行了检测和分析。1996年11月和12月，周博士和船本博士一起前往日本的东广岛、新泻、凉津、磐城、千叶和日南进行了百合科和毛绒科植物的调查和收集，并将这些活体标本带回了中国。并在广岛大学理学院植物染色体与基因库实验室掌握植物组织培养技术。1997年2月，洪教授到东广岛考察，收集了百合科和毛茅科的一些物种，并与近藤教授讨论了进一步的合作研究。

In early August, 1996, Prof.Kuroki and Mr.Kokubugata went Jilin and Heilongjiang Provinces, China. Prof, Kuroki found some dioecious Rumex and Allium during their trip, and brought these live collections back to Japan for DNA conservation of Rumex. Mr.Kokubugata collected Dendranthema oreastra which is an endangered species in Mt.Chengbai, Jilin Province and performed molecular cytology for conservation of species diversity. Moreover, he sowed surface-sterilized seeds of this species on 1/2 MS agar medium in vitro. Prof.Shimada and Dr.Funamoto made a field trip to Sichuan Province, China in early August, 1996, and collected some species of Primula and Saxifraga. Prof.Shimada succeeded in in vitro conservation of genetic resource of Primula by tissue-culture from an explant of leaf. Dr.Funamoto cytologically analyzed species diversity of collected species. Prof.Kondo and Prof.Hizume made a field trip to Shaanxi Province, China in early October, 1996. Prof.Kondo collected some species of Chrysanthemum sensu lato, and performed molecular cytotaxonomy for analysis of species diversity. Moreover, he succeeded in vitro conservation of gene resources by tissue-culture. Prof.Hizume collected some species of Pinus, Picea, Abies and Ephedra and then, detected and analyzed their chromosome variation with fluorescent differential staining methods and fluorescent in situ hybridization.Dr.Zhou made a trip to Higashi-Hiroshima, Niigata, Ryotsu, Iwaki, Chiba and Nichinan, Japan with Dr.Funamoto for investigations and collections of Liliaceae and Ranunculaceae, in November and December, 1996, then, brought those live collections back to China. Moreover, he mastered techniques of plant tissue-culture in Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University. Prof.Hong made a trip to Higashi-Hiroshima, in February, 1997, and collected some species of Liliaceae and Ranunculaceae, and discussed further cooperative researches with Prof.Kondo.