Molecular Genetics on Origin, Distribution and Control of Lyme Disease

1)用EcoRV消化莱姆病螺旋体基因组DNA，与23 SrRNA基因特异性探针杂交，将莱姆病螺旋体分为I-X等10种类型。2）根据OspC蛋白和鞭毛蛋白基因的碱基序列，核糖体II型，日本分离株Ⅳ ~ Ⅵ型为伽氏疏螺旋体。3）日本LD的主要病原体为B.garinii和B.afzelii，主要媒介为全沟硬蜱。主要宿主为黑线姬鼠、红腹姬鼠和候鸟，主要宿主为黄鹂和黄鹂。4）根据抗OspA单克隆抗体的反应性，将日本LD型疏螺旋体分为11个血清型。欧美分离株可分为7个血清型。5）在俄罗斯远东地区，全沟硬蜱携带率为26.6%（225/846）。B.garinii和B.afzelii分离自姬鼠属、鼠属、Tamias和Sorex属的硬蜱和啮齿动物，它们的比例分别约为80%和10%。在俄罗斯远东地区的分布模式与日本的分布模式非常相似。6）与Simon博士合作，从卵形硬蜱中分离出的日本疏螺旋体引起SCID、C3 H/HeN和ddY小鼠关节炎。7）从Tanuki、I.turdus和I. clumanus中分离出的3个疏螺旋体分别被认为是新种。具有38 kDa鞭毛蛋白和1套23/5S rRNA基因，来自全沟硬蜱，与回归热病原体关系较近，命名为宫本双歧杆菌。9）1996年1月22/23日在美国文图拉举行了合作研究会议，出席会议的有约翰逊、Baranton、Simon、Wilske、Fukunaga、Masuzawa和Yanagihara证实了这项联合研究的成功。

1) Genomic DNAs of Lyme disease (LD) borreliae were digested with EcoRV of Hinc II and hybridized with probes specific for 23S rRNA genes and borreliae were classified into 10 types, I-X.2) From base sequences of OspC protein and flagellin genes, ribotype II,and IV-VI of Japanese isolates were classified as B.garinii.3) Main causative agents of Japanese LD were B.garinii and B.afzelii and main vector was I.Persulcatus. Main reservoir was Apodemus speciosus, and Clethrionomys rufocanus bedfordiae and migrating birds, Turdus chrysolaus and Emberiza spodocephala playd as reservoirs.4) Japanese LD borreliae were classified into 11 OspA serotypes based on reactivities to anti-OspA monoclonal antibodies. European and American isolates were classifed into 7 OspA serotypes. Only 1 type were identical each other.5) In Far East Russia, I.persulcatus harbored the organism at 26.6% (225/846). B.garinii and B.afzelii were isolated from ixodid ticks and rodents, genera Apodemus, Clethrionomys, Tamias, and Sorex, and their ratios were about 80% and 10%, respectively. No B.burgdorferi sensu stricto was found. Distribution pattern of Borrelia in Far East Russia was closely similar to that in Japan.6) B.japonica isolated from I.ovatus caused arthritis in SCID,C3H/HeN and ddY mice with collaborative works with Dr.Simon.7) Three borreliae isolated from I.tanuki, I.turdus and I.clumnae were suggested as new species, respectively.8) Borrelia, having 38kDa flagellin and 1 set of 23/5S rRNA genes, from I.persulcatus was distantly close to relapsing fever agent and named as B.miyamotoi.9) Meeting on the Cooperative Research was held in Ventura, U.S.A.on 22/23 Jan.1996 under the attendance of Drs.Johnson, Baranton, Simon, Wilske, Fukunaga, Masuzawa and Yanagihara and confirmed the success of this Joint Research.