Hormonal Control of osmoregulation in euryhaline fis

武井拜访了曼彻斯特大学的Balment博士，对比目鱼进行了以下实验：(1)向比目鱼体内注射血管松弛药物罂粟碱，测量饮酒率、血浆血管紧张素II和血管催产素水平的时间进程。此外，还观察了血管紧张素II抑制对罂粟碱对鳗鱼饮酒率和血浆血管生成素水平的影响。(2)首次对鳗鱼体内的心房利钠肽(ANP)进行了测序。方法：1.将人工合成的鳗鱼心钠素注射到欧洲鳗鱼体内，观察其饮水率和血浆血管催产素水平变化的时程。Hyodo还拜访了Balment博士，并进行了以下实验。(1)他发现虹鱼转移到海水中后，血管催产素mRNA的表达减少，而Balment博士发现，转移到海水中后，虹鱼体内的血管催产素水平上升。因此，他们共同在比目鱼身上进行了类似的实验。(2)Northern印迹分析检测了鲑鱼脑室旁核血管催产素mRNA表达的时程变化。滨野拜访了圣安德鲁斯大学的哈森博士，并进行了以下实验。(1)她之前曾与哈森博士合作，研究角鱼血管紧张素II的升压作用，发现升压作用的机制与其他动物截然不同。她还用离体角鱼主动脉环检测了同源C型利钠肽的血管松弛活性。

Takei visited Dr.Balment of Manchester University to conduct following experiments using flounder.(1) Papaverine, a vasorelaxant drug, was injected into the flounder, and the time course of drinking rate, plasma angiotensin II and vasotocin levels were measured. The effect of angiotensin II inhibition on drinking rate and plasma vasotocin level after papaverine was also examined.(2) We sequenced eel atrial natriuretic peptide (ANP) in eels for the first time in fishes. Synthetic eel ANP was injected into European eels, and the time course of change in drinking rate and plasma vasotocin level was examined.2. Hyodo also visited Dr.Balment and conducted the following experiments.(1) He found that the expression of vasotocin mRNA decreased after transfer of rainbow trout to seawater, and Dr.Balment found that plasma vasotocin level in the rainbow trout increased after transfer to seawater. Thus, they worked together to conduct similar experiments in the flounder.(2) The time course of change in vasotocin mRNA expression in the paraventricular nucleus of the trout brain was examined by Northern blot analysis.3. Hamano visited Dr.Hazon of University of St.Andrews and conducted the following experiments.(1) She had worked with Dr.Hazon previously for the vasopressor effect of angiotensin II in the dogfish, and found that mechanism of the pressor effect was quite different from that of other animals. She further analyzed the mechanism using an isolated aortic ring of dogfish.(2) She also examined the vasorelaxant activity of homologous C-type natriuretic peptide using isolated aortic ring of dogfish.