On the Relation to Genetical Factors of Strain-Difference of Anesthetic Sensitivities and Brain-distribution of Neurotransmitters in ddN and C57BL mic

1)麻醉药敏感性的遗传学分析：以异氟醚（ISO）和安氟醚（ENF）的ED_（50）作为ddN和C57 BL小鼠、相互杂交小鼠及其回交后代的右反射丧失指标。结果表明：（1）ddN和C57 BL小鼠对ENF和ISO的敏感性存在差异。C57 BL小鼠比ddN小鼠更敏感。与1990年的1.38倍相比，这一差距缩小到1.18 - 1.26倍。与ddN小鼠的麻醉剂抗性相关的遗传因素可能通过近亲繁殖的继续而丢失。(2)父母和F1杂种的数据的多元回归分析表明，虽然母体因素和常染色体上的因素与两种麻醉剂有关，但X染色体上的因素是ENF特异性的。在回交的同胞后代中麻醉剂需求的广泛变化表明多因素遗传。所得的回归方程并不总是预测 ...更多信息 回交同胞后代中抽动要求。这些差异可能是由于相关基因的上位性相互作用。结论：ddN和C57 BL小鼠对ENF和ISO麻醉剂的需要量与多种不同的遗传因素有关。2）ddN、C57 BL及其杂种（F1）小鼠脑神经递质和受体的免疫组织化学分析。(1)血清素：海马CA 1、CA 2、CA 3区的降钙素免疫反应神经纤维密度为ddN <C57 BL，海马齿状回的降钙素免疫反应神经纤维密度为ddN > C57 BL。(2)后脑磷脂：海马苔藓纤维的免疫反应性为ddN > F1 '> F1 > C57 BL。(3)神经肽Y：结果表明，在顶颞叶皮质中，ddN <C57 BL，在视交叉上核中，ddN、F1、F1' <C57 BL，在室旁核中，ddN、F1、F1' <C57 BL，在三叉神经脊束核中，ddN <C57 BL，在颈后核中，ddN <C57 BL。(4)多巴胺：黑质纹状体多巴胺能通路在ddN中比在C57 BL中更丰富。ddN的黑质（substantia nigra）比C57 BL的大30%左右。（纹状体）的ddN大于C57 BL，酪氨酸羟化酶免疫反应阳性结构的染色强度，这可以解释为末端的密度，在ddN中的表达高于C57 BL。(5)谷氨酸受体：AMPA-选择性谷氨酸受体的定位进行了检查，使用抗血清对亚基：GluR 1，GluR 2/3，和GluR 4。在海马CA 1区和齿状回，C57 BL的GluR 1和GluR 2/3的分布较ddN的密集，顶叶大脑皮质的GluR 2/3抗血清染色强度较ddN的强。(The图像分析正在进行中。）少

1) On the genetical analysis of anesthetic sensitivities : ED50s of Isoflurane (ISO) and Enflurane (ENF) were estimated as an index of loss of righting-reflex in ddN and C57BL mice, mutual hybrid mice and their backcross progenies. The results are as follows ;(1) There is the difference of ENF and ISO sensitivities in ddN and C57BL mice. C57BL mice is more sensitive than ddN mice. This difference decreases into 1.18 - 1.26 times compared with the value of 1.38 times in 1990. A genetical factors related to anesthetic resistance in ddN mice may be being lost by means of the continuation of inbreeding.(2) Multivariate regression analysis of parents' and F1 hybrids'data revealed that, while maternal factors and factors on autosomes were related to both anesthetics, the factors on the X chromosome were ENF specific. A wide variation i anesthetic requirements among backcross siblings progeny suggested multifacorial inheritance. The regression equations pbtained did not always predict anesthe … More tic requirement in backcross siblings progeny. These discrepancies may be due to the epistatic interaction of related genes. We conclude that multiple but different genetic factors are involved in determining ENF and ISO anesthetic requirements in ddN and C57BL mice.2) Immunohistochemical analysis of brain neurotransmitters and receptors in ddN,C57BL and their hybrid (F1) mice.(1) Serotonin : The density of serotonin-immuno reactive nerve fibers is ddN < C57BL in hippocampus CA1, CA2 and CA3, ddN > C57BL in dentate gyrus of hippocampus.(2) Metencephalin : The immunoreactivity of mossy fiber of hippocampus is ddN > F1'> F1 > C57BL.(3) Neuropeptide Y : The immunoreactive nerve cell is ddN < C57BL in parietotemmporal cortex and the immunoreactivity is 2 x ddN,F1, F1' < C57BL in suprachiasmatic nucleus, F1, F1' < ddN < C57BL in paraventricular nucleus, F1 < F1' and ddN < C57BL in spinal trigeminal nucleus, ddN < C57BL in cervical dorsal nucleus.(4) Dopamine : The nigrostriatal dopaminergic pathway is more abundant in ddN than C57BL.The number of the dopaminergic neurons in the origin (substantia nigra) of ddN is about 30% larger than that of C57BL.The area of the terminal field (striatum) of ddN is larger than C57BL,and the staining intensity of tyrosine-hydroxylase immunoreactive structure, which can be interpreted as the density of the terminal, is higher in ddN than that of C57BL.(5) Glutamate receptor : The localization of AMPA-selective gluamate receptor were examined using the antiserum made against the subunits : GluR1, GluR2/3, and GluR4. In the hippocampal CA1 and dentate gyrus, the distribution of GluR1 and GluR2/3 of C57BL are more dense than that of ddN.The staining intensity against the GluR2/3 antiserum in the parietal cerebral cortex is higher in C57BL than ddN.(The image analysis is in progress.) Less