Culture of piroplasms using some components of reticulocytes for the purpose of cell-free culture of the parasite.

目的：探讨促进犬网织细胞中巴贝斯虫增殖的因素。将巴贝斯虫寄生虫与遗传性谷氨酸浓度高的犬红细胞或谷氨酸浓度低的犬红细胞一起培养。巴贝斯虫寄生在含有网织红细胞中存在的几种物质的培养基中培养。在含有高谷氨酸的红细胞中观察到寄生虫的最高增殖。(1)用葡萄糖或谷氨酸培养时，寄生虫的增殖率较高。(2)将寄生虫与含有网织红细胞溶血沉淀物的重封细胞一起培养，可促进寄生虫的增殖。（结论）这些结果表明，谷氨酸和葡萄糖都是gibsoni生长所必需的，胞内细胞器可能是促进gibsoni在犬网织细胞中增殖的重要因素。

To clarify the factors which enhance the multiplication of Babesia gibsoni in canine reticulocytes in vitro.1. Babesia parasites were cultured together with canine erythrocytes with hereditary high concentration of glutamate, or cells with low glutamate.2. Babesia parasites were cultured in the medium containing several materials which present in reticulocytes.(Results)1. The highest multiplication of the parasites was observed in the erythrocytes containing high glutamate.2.(1) A high multiplication of the parasites was observed when the parasites were cultured with glucose or glutamate.(2) The multiplication of the parasites was increased when the parasites were cultured together with resealed cells containing sediments of hemolysate of reticulocytes.(Conclusion)These results indicate that both glutamate and glucose are necessary for growth of B.gibsoni, and that the intracellular organelles may be an important factor which enhance the multiplication of B.gibsoni in canine reticulocytes.