Infectious disease, and hepatic and gastrointestinal disease in Japan and China

由于目前对人肝脏二肽基肽酶II （DPP II）的性质知之甚少，我们试图确定其分子量、动力学参数、氨基酸序列和cDNA结构等特征。然而，由于人体肝脏不容易获得，作为初步实验，我们尝试从人精浆中纯化DPP II。用DEAE-Sephacel、Superdex 200和Phenyl-Sepharose在SDS-PAGE上纯化DPP II至均质。我们还测定了它的分子量（Mr）、n端氨基酸序列及其在人前列腺中的免疫组化定位。纯化酶的Mr为140,000。该酶优先裂解Ala-、Phe-、Leu-和Lys-Ala-MCAs等底物。该蛋白强烈染色于前列腺腔细胞。此外，该酶的氨基酸序列和cDNA结构表明，DPP II是一种氨基肽酶，即丙烯酰氨基肽酶（alanyl aminopeptidase， AAP）。在我们利用人肝脏进行的初步实验和上述数据的基础上，我们还计划从人肝脏中纯化酶，并在中日研究小组的合作下进行表征。口腔中嗜酸性细菌数量多、种类多，给致病菌的分离带来了很大的困难。在本研究中，我们将日本感染与中国感染进行比较，以建立有效的药物治疗方法。样本采集于吉林大学附属医院感染治疗不足的患者。对样品进行培养，并进行细菌学检查。结果，收获了葡萄球菌、肠球菌、链球菌、胃球菌、胃链球菌、棒状杆菌、乳酸杆菌、诺卡菌、牙霉菌和细菌体。用生物测定法测定了这些细菌对某些抗生素的敏感性。然后，敏感抗生素是头孢菌素和青霉素。这些结果与我们之前在我们部门检查的结果非常相似。少

On the reason that little is known the properties of human liver dipeptidyl peptidase II (DPP II), we tried to determine the characteristics incliding molecular weight, kinetic parameters, amino acid sequence and cDNA structure. However, since it is not so easy to get human liver, as a preliminary experiment, we tried the purification of DPP II from human seminal plasma. The DPP II was purified to homogenity on SDS-PAGE by DEAE-Sephacel, Superdex 200 and Phenyl-Sepharose. We also determined its molecular weight (Mr), N-terminal amino acid sequence and its immunohistochemical localization in human prostatic gland. The Mr of the purified enzyme was 140,000. The enzyme preferentially clevaged the substrates such as Ala-, Phe-, Leu- and Lys-Ala-MCAs. The protein was strongly stained the luminal cells in prostatic gland. Furthermore, the amino acid sequence and cDNA structure of the enzyme indicated that DPP II in human seminal plasma is an aminopeptidase, namely alanyl aminopeptidase (AAP) … More . On the basis of our preliminary experiment using human liver and the above data, we are also planing to purify the enzyme from human liver, and to characterize under the colaboration between Japanese and Chinese research groups.It is very difficult to isolate of pathogenic bacteria because of there are many number of and many sort of mesophilic bacteria in mouth. In this study, we compared with the infection in Japanese and the infection in Chinese to establish a effective medicinal therapy.The samples were collected from the patients who were undertreating for infection in Chirlin University Hospital. The samples were cultured and tested by basteriological examination.In result, Staphylococcus, Enterococcus, Streptococcus, Peptococcus, peptostreptococcus, Corynebacterium, Lactobacillus, Norcardia, Odontomyces and Bacterionema were harvested. Susceptibility of these bacteria to some antibiotics was tested by bioassay. Then, susceptible antibiotics were Cephalosporin and Penicillin.These results were very similar to our results which were examined in our department before. Less