Development of Central Neurons System and placental fuction.

各种器官的发育在胎盘形成后立即开始。我们之前的研究表明，大鼠羊水中含有具有免疫反应性的胎盘乳原样分子，其分子大小和pI值与大鼠真实胎盘乳原（PLs）不同。在本研究中，利用二维SDS凝胶电泳进一步表征了羊水中存在的免疫反应性PLs，我们证实了妊娠第12天收集的大鼠羊水含有两种pl样分子，暂定为A1 （MW 75 kDa, pI4.6）和A2 （MW 99-102 kDa, pi5.3 -5.4）。PLs具有与催乳素（PRL）受体结合的能力，其靶组织之一是成年动物的大脑。因此，我们尝试在胎鼠大脑中检测PRL受体（PRL- r） mrna，该基因的表达在发育中的胎鼠大脑中很早就被检测到。为了研究n-寡糖在大鼠胎盘乳原- i嵌合中的分子作用，我们在COS7细胞中制备了重组PL-Im （recPL-Im）和3个recPL-Im突变体。结果表明，PL-Im在Asn79和Asn128位点均发生n糖基化。此外，这两个n -链寡糖也参与了其生物活性。有趣的是，PL-Im结合PRL-R和激活JAK2的能力似乎与n -糖基化无关。我们还发现了一个新的基因，4P16，它与神经细胞的增殖有关。PL-Im在Nb2细胞系中也能刺激该基因的表达。为了识别神经发生过程中在大脑中特异性表达的分子，我们试图产生单克隆抗体，以识别具有独特时间表达模式和分子特征的分子。其中一种抗体Mab3C8识别了胎儿大脑中富集的100 kda抗原。这种100 kda抗原在胎儿时期（从E12到E20）持续表达，并在出生后2天无法检测到。通过产生抗体，发现了胎儿大脑特有的分子，一种分子量为100 kDa （FOG100）的o链唾液糖蛋白。除了这些结果外，我们还发现胎儿大脑表达了三种孤儿受体NGFI-B，Rev-erbAa， Rev-erbAb的mrna。最后，我们在胎盘和大脑中发现了几种具有不同PI值的G蛋白β sabunit形式。少

Development of various organs begins immediately after initiation of placentation. We previously showed that rat amniotic fluid contains immunoreactive placental lactogen-like molecules with different molecular sizes and pI values from the authentic placental lactogens (PLs) in the rat. In the present study, immunoreactive PLs residing in the amniotic fluid were characterized further by two-dimensional SDS gel electrophoresis and we confirmed that amniotic fluid collected from rats on day 12 of pregnancy contains two PL-like molecules, tentatively called A1 (MW 75 kDa, pI4.6) and A2 (MW 99-102 kDa, pI 5.3-5.4). PLs have ability to bind to prolactin (PRL) receptor and one of the target tissues for PLs is the brain in adult animals. Therefore, we tried to detect PRL receptor (PRL-R) mRNAs in fetal rat brains, and the gene expression is detected very early in developing fetal brain. To evaluate a role for N-linked oligosaccharides in the molecular action of rat placental lactogen-I mosaic … More (PL-Im), recombinant PL-Im (recPL-Im) and three recPL-Im mutants were produced in COS7 cells. It became evident that PL-Im was N-glycosylated at both Asn79 and Asn128. In addition, the two N-linked oligosaccharides which are involved in its biological activity. Interestingly, the ability of PL-Im to bind PRL-R and activate JAK2 appears to be independent of the N-glycosylation. We also found a novel gene, 4P16, which is related to the proliferation of neural cells. The gene expression is also stimulated by PL-Im in the Nb2 cell line. With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became undetectable two days after birth. The molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular weight of 100 kDa (FOG100), was found by generating an antibody. In addition to these results, we also found that the fetal brain express the mRNAs for tree orphan receptors, NGFI-B,Rev-erbAa, Rev-erbAb. Finally, we have idenstified several forms of G protein beta sabunit with different PI values in the placenta and brain. Less