DESIGNING OF HEALTHFUL ENVIRONMENT FOR HUMAN LIFE-A BASIC STUDY TO CLARIFY THE PREVENTIVE EFFECT OF AN ANTI-STREES VITAMIN,VITAMIN C ON THE COMMON CHRONIC DISEASE OF ADULT PEOPLE

左旋抗坏血酸（阿萨）是一种水溶性抗氧化剂，也是灵长类动物的必需营养素，由于其自身缺乏生物合成能力。被动吸烟，暴露于香烟的侧流烟雾中，被认为会增加非吸烟者患某些疾病的风险，因为香烟的侧流烟雾比主流烟雾中含有更多对身体有害的有机化合物和自由基。为了阐明阿萨对香烟烟雾化学应激的生物防御作用，本研究从被动吸烟的角度研究了阿萨代谢和药物代谢酶活性。将合成阿萨的Wistar雄性大鼠和不合成阿萨的ODS大鼠分为对照组（C组）和实验组（T组）。T组每天暴露于香烟侧流烟雾2h。实验结束后，用高效液相色谱法测定大鼠肝脏和其他组织中阿萨的含量，结果表明，香烟烟雾暴露后，Wistar大鼠和ODS大鼠各组织中的阿萨含量和药物代谢酶活性均升高。推测大鼠体内合成阿萨，当阿萨作为抗氧化剂与某些自由基反应或支持提高药物代谢酶的活性对抗化学应激时。在ODS大鼠暴露于香烟烟雾的情况下，一个意想不到的增加阿萨含量在各种组织中观察到，这种明显的增加可能是由于阿萨代谢的一些适应性的化学应激，由于香烟烟雾。因此，被动吸烟对大鼠和ODS大鼠的阿萨代谢和药物代谢酶活性均有明显影响。

L-ascorbic acid (AsA), known as Vitamin C is a water-soluble antioxidant and an essential nutrient for the primate, because of their deficiency to biosynthesize it. Passive smoking, exposed to sidestream smoke from cigarette, is said to increase the risk of certain disease in nonsmokers, since sidestream from cigarette have much more harmful organic compounds and free radicals for body than mainstream smoke from cigarette. In this study, in order to clarify the bio-defense function of AsA against chemical stress from cigarette smoke, the AsA metabolism and the activities of drug-metabolizing enzyme were studied from the standpoint of the effect of passive smoking. Wistar male rats, which synthesized AsA,and ODS rats, which chould not synthesize AsA,were divided into two group, the control (C group) and the test (T group) groups. The animals in T group were exposed to sidestream smoke from cigarette for 2 hours everyday. After the experimental period, the AsA concentration in liver, and other tissues were measured by HPLC.When exposed to cigarette smoke, AsA levels in various tissues and activities of drug-metabolizing enzymesincreased in Wistar rats and ODS rats. It was speculated that AsA was synthesized in rats, when AsA was consumed to react with some radicals as an antioxidant or to support in raising the activities of drug-metabolizing enzymes against chemical stress. In the case of ODS rats exposed to cigarette smoke, an unexpected increase of AsA contents in various tissues was observed, and this apparent increase might be the result of some adaptation in AsA metabolism against chemical stress due to cigarette smoke. Therefore, it was concluded that the passive smoking gave a considerable effect on both AsA metabolism and activities of drug-metabolizing enzymes in rats and ODS rats.