Expression of basic FGF,FGF receptor, TGF-beta, and VEGF in experimental choroidal neovascularization

脉络膜新生血管形成（choroidal neovascularizaion，ChNV）的发生和消退可能受生长因子和细胞因子的调节。为揭示碱性成纤维细胞生长因子（B FGF）、FGF受体、转化生长因子（TGF）、血管内皮生长因子（VEGF）在色素性大鼠强光凝（PC）诱发的ChNV中的存在和定位，采用原位杂交和免疫组织化学方法，研究了它们的mRNA表达。以大鼠B FGF、FGF受体、TGF-β_1、小鼠TGF-β_2、TGF-β_3、VEGF cDNA为探针，制备反义和正义核酸探针。在PC后的视网膜和脉络膜切片上进行原位杂交，在正常大鼠视网膜神经节细胞层中检测到碱性FGF、FGF受体、TGF-β_1、TGF-β_2和VEGF的mRNA，而未检测到TGF-β_3。在PC后的ChNV发展阶段，视网膜色素上皮细胞和血管内皮细胞表达B FGF、FGF受体和VEGF mRNA。TGF-β_1和TGF-β_2 mRNA的表达。这些结果提示内源性B FGF和VEGF促进了脉络膜新生血管的形成，TGF-β_1和β_2可能参与了体内ChNV的调节。

Development and regression of choroidal neovascularizaion (ChNV) may be regulated by growth factors and cytokines. To reveal existence and localization of basic fibroblast growth factor (b FGF), FGF receptor, transforming growth factor (TGF), vascular endothelial growth factor (VEGF) in ChNV,we demonstrated expression of mRNAs of them with in situ hybridization method and immunohistochemical study.ChNV were produced in pigmented rat with intense photocoagulation (PC). Antisense and sense riboprobes were prepared from rat b FGF,FGF receptor, TGF-beta_1, mouse TGF-beta_2, TGF-beta_3, VEGF cDNAs. In situ hybridization was performed on sections of the retina and choroid following PC.Basic FGF,FGF receptor, TGF-beta_1, TGF-beta_2, and VEGF,mRNAs were detected in the ganglion cell layr in normal rat retina, though TGF-beta_3 was not detected. In developing stage of ChNV after PC,expression of b FGF,FGF receptor and VEGF mRNAs were marked in the retinal pigment epithelial cells, and vascular endothelial cells. Expression of TGF-beta_1 and TGF-beta_2, mRNAs were also detected. However, these factors were no longer detected in regressing stage of ChNV.These results suggest endogenous b FGF,and VEGF promotes development of choroidal neovascularization, and TGF-beta_1 and beta_2 appears to be associated in regulation of ChNV,in vivo.