Development of a multi-agent system for plant biocommunication

在需要稳定高效生产的植物工厂中，为了实现最大产量和最高质量，精确测量植物的直接反应是至关重要的。然而，植物生理与其受控环境之间的关系尚不清楚。本研究的目的是开发一个多智能体系统来测量植物在几个方面的反应。利用稳定同位素，可以同时测量光合作用和呼吸作用。此外，本文还分析了激光诱导下植物的荧光响应，并利用热像仪检测了植物叶片的温度变化规律。已经建立了一个叶片室，并使用稳定同位素^<13>CO_2对玉米和马铃薯叶片进行了测试。假设不产生使用^<13>CO_2的光合产物，进行光合作用和呼吸作用的分离，即同时测量光合作用和呼吸作用。从这个方法可以清楚地看到光呼吸作用。利用相同的系统，对马铃薯和甘薯进行了测量，以确定净光合作用和总光合作用。总光合作用的测量尚未完全明确。研制了植物激光诱导荧光检测系统。以菠菜为研究对象，叶绿素a/b比值的下降与植株年龄之间存在相关性。对番茄幼苗进行了水分胁迫试验。685nm和740nm的峰值响应有一定的变化趋势，但不清楚。利用热像仪测量甜瓜幼苗的水分胁迫，给出叶片的温度模式。并对图案的绿色成分进行了分析。增温与g值下降相关性较好，表现出水分胁迫趋势。

It is essential to mesure precisely direct reaction from plants, in order to achieve the maximum production and highest quality of plants in a plant factory where stable and high efficiency production is required. However, it has not yet been cleared that the relationship between plant physiology and its controlled environments. The purpose of the present study is to develop a multi-agent system to measure plant responses in several aspects. Using stabel isotopes, the simultaneous measurement of photosynthesis and respiration has been developed. Furthemore, the plant fluoresence response induced by a laser beam has been analyzed and temperature pattern of leaves by thermograph was used to detect water stress or plants.A leaf chamber has been built and corn and pothos leaves have been tested by using a stable isotope ^<13>CO_2. The separation of photosynthesis and respiraton, that is, simultaneous measurement of photosynthesis and respiration was conducted, assuming the photosyntheis product using ^<13>CO_2 did not occurer. From this method, photoresipiration was clearly shown. Using the same system, the measurement of potato and sweet potato has been conducted to find net and gross photosynthesis. The measurement of gross photosynthesis has not been completely cleared.The system to detect laser induced fluorescence from plants was developed. Using spinach plants, it has been cleared that a correlation between decreasing values of the ratio of chlorophyll a/b and plant age. Water stress of tomato seedlings was tested. The peak responses at 685nm and 740nm show some trend but it is not clear.Water stress of melon seedlings was measured by a thermograph which gave temperature pattern of the leaves. Green component of the pattern was also analyzed. Temperature in crease and G-value decrease are well corelated and they showed water stress tendency.