Development of Masspropagation Systems for Tissue Culture

组织培养大规模繁殖系统的开发

• 批准号: 02556035
• 负责人: TAKAKURA Tadashi
• 金额: $ 10.24万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02556035/
• 关键词: Tissue Culture; Somatic Embryo; Relative Humidity; Gas Exchange; 水ポテンシャル; 換気; 培養苗; 大量培養; 育苗システム

Automatization of the massproduction of tissue culture seedlings including somatic embryogenesis is one of the problems to be solved urgently. A prototype system was constructed and tested for automation of selecting procedure of appropriately sized callus by sieving in somatic embryo production. Back washing the mesh was found to be effective for preventing the mesh blockage. Although the system performance did not reach that of the manual sieving, the experiment suggested that the basic ideas developed in this study would be effective for the future system. The application of artificial vision system which demands for the detailed knowledge of morphology of somatic embryo is promising methodology in somatic embryogenesis.Acclimatization to prevent glassification mainly due to wet condition is needed for liquid culture which is very popular method. Nutrient mist culture which brings dryer environment was investigated for potato plantlet growth. Nutrient supply for 1 min. per a 10 min. cycle bought about the largest increase of fresh and dry weights and the best root development. It is expected that further optimization of the mist supply variations may bring about more efficient propagation, although some scattering of data was observed.Tomato plantlets were cultured for 25 days in four different relative humidity conditions to investigate the transpiration and net photosynthetic rates. Net photosynthetic rate per unit leaf area increased with increasing relative humidity. An experiment was conducted to compare the multiplication and growth of the Cymbidium PLB under photoautotrophic and photomixotrophic conditions. Total dry weight of the PLB was greater in photomixotropic condition.Water and nutrient supply to hydroponic tomato plants in a greenhouse id identified by using neural nets. By using 8 hour input data and single hidden layer which consists of 4units, the amount of water and nutrient supply was calculated well.

组培苗包括体细胞胚胎发生的大量生产的自动化是目前亟待解决的问题之一。构建了一个原型系统，并对该系统进行了测试，以实现体细胞胚生产中筛选合适大小愈伤组织过程的自动化。发现回洗网对于防止网堵塞是有效的。虽然系统的性能没有达到人工筛选，实验表明，在这项研究中开发的基本思想将是有效的，为未来的系统。人工视觉系统的应用需要对体细胞胚的形态学有详细的了解，是体细胞胚发生的一种有前途的方法，而液体培养是一种非常流行的方法，它需要进行驯化以防止主要由于潮湿条件引起的玻璃化。研究了营养液雾培对马铃薯试管苗生长的影响。每10 min循环供肥1 min，鲜重和干重的增加量最大，根系发育最好。预计进一步优化的薄雾供应的变化可能会带来更有效的传播，虽然一些分散的数据进行了观察。番茄试管苗培养25天，在4个不同的相对湿度条件下，研究蒸腾和净光合速率。单位叶面积净光合速率随相对湿度的增加而增加。比较了光自养和光混合营养条件下大花蕙兰原球茎的增殖和生长情况。光合混合营养条件下原球茎的总干重较大。利用神经网络对温室水培番茄植株的水分和养分供应进行了识别。采用8小时的输入数据和由4个单元组成的单隐层，较好地计算了土壤水分和养分的供应量。

项目成果

IBARAKI,Y.,and KURATA,K.: "Micropropagationby nutirent mist supply:Effects of amist supply period on patato plantlet growth." 131-135 (1991)

IBARAKI,Y. 和 KURATA,K.：“通过营养雾供给进行微繁殖：雾气供给期对马铃薯幼苗生长的影响”。

Tanaka,K., Fujiwara,K., and Kozai,T.: "Effect of relative humidity the culture vessel on the transpiration and net photosynthetic rates of potato plantlets in vitro" Acta Horticulturae. 319. 59-64 (1992)

Tanaka,K.、Fujiwara,K. 和 Kozai,T.：“培养容器相对湿度对马铃薯试管苗蒸腾作用和净光合速​​率的影响”《园艺学报》。

IBARAKI,Y.,IIDA,Y.,and KURATA,K: "Effects of air currents on gas exchange of cultore vessels." A cta Horticulturae. 319. 221-224 (1992)

IBARAKI,Y.、IIDA,Y. 和 KURATA,K：“气流对培养皿气体交换的影响”。

Kirdmanee,C., Kubota,C., Jeong,B.R., and Kozai,T.: "Photoautotrophic multiplication of cymbidium protocorm-like bodies" Ibid.319. 243-247 (1992)

Kirdmanee,C.、Kubota,C.、Jeong,B.R. 和 Kozai,T.：“蕙兰原球茎样体的光自养繁殖”同上 319。