Development of a plant-factory to produce useful materials of scarcity value.

开发植物工厂来生产具有稀缺价值的有用材料。

• 批准号: 10460116
• 负责人: TAKAKURA Tadashi
• 金额: $ 7.81万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460116/
• 关键词: Tissue Culture; Useful Material; Plant Factory; Bioreactor; Agave pacifica; CAM model; Simulation; リュウゼッラン

As a major source of hard fiber and a potential source of certain steroids, Agave pacifica is of considerable economic importance. Agave pacifica has three natural ways of reproduction ; 1) bulbils which are young plantlets that occur on the inflorescence ; 2) rhizonmes which are underground stems generated from the base of the stems and at the end of which a new plant, or ramet, emerges ; and 3) seedling establishment which is extremely rare for Agave pacifica, even though each inflorescence can produce several thousands of seeds. Only a very small percentage of the Agave pacifica is propagated by seedlings. The specific objectives of the present study are to develop a plant-factory style production system for Agave pacifica, to cultivate its seedlings from cullus, and to develop a photosynthesis model by Simulink.The bioreactor system has been successfully designed and constructed. Two kinds of experiment has been conducted using cullus generated by tissue culture and seeds to grow seedlings using this bioreactor. The survival rate of the growth from cullus was not high and growth variation of seedlings from seeds was relatively large. The photosynthesis model has been developed and the basic model behavior was analyzed. Further study is needed to complete the model study.

作为硬纤维的主要来源和某些类固醇的潜在来源，龙舌兰具有相当大的经济重要性。龙舌兰有三种自然的繁殖方式：1）珠芽，是在花序上出现的幼小植株; 2）根茎，是从茎的基部产生的地下茎，在其末端出现新的植物或分株; 3）幼苗建立，这对于龙舌兰来说是极其罕见的，即使每个花序可以产生数千粒种子。只有很小比例的龙舌兰是通过幼苗繁殖的。本研究的具体目标是建立一个植物工厂式的龙舌兰生产系统，培养其苗从culb培养，并建立了一个光合作用模型的Simulink。生物反应器系统已成功地设计和构建。利用组织培养产生的愈伤组织和种子在该生物反应器上进行了两种实验。愈伤组织生长的成活率不高，种子成苗的生长变异较大。建立了光合作用模型，分析了模型的基本行为。需要进一步的研究来完成模型研究。

项目成果

高倉直: "CAM植物光合成のシミュレーション"日本生物環境調節学会講演要旨集. (発表予定). (2001)

Nao Takakura：“CAM 植物光合作用的模拟”日本生物与环境调节学会会议记录（预定报告）（2001 年）。

池永、高倉: "Agave Pacificaのカルス培養とバイオリアクタによる栽培" 日本生物環境調節学会講演要旨集. 7月(発表予定). (1999)

Ikenaga, Takakura：“Agave Pacifica 的愈伤组织培养和使用生物反应器的培养”日本生物和环境调节学会 7 月摘要（待发表）。

Shimomachi,T.: "Nondestructive detection of water stress in tomato Plants by microwave sensing."Environ.Control in Biol.. 39. 17-26 (2001)

Shimomachi,T.：“通过微波传感对番茄植株水分胁迫进行无损检测。”Environ.Control in Biol.. 39. 17-26 (2001)

池永、高倉: "Agave Pacificaの培養"日本生物環境調節学会講演要旨集. 8月(発表予定). (2000)

Ikenaga, Takakura：“龙舌兰太平洋文化”日本生物与环境调节学会摘要（2000 年）。

Shimomachi, T., N.K.Okamura, T.Takemasa, and T.Takakura.: "Nondestructive detection of water stress in tomato Plants by microwave sensing."Environ.Control in Biol.. 39. 17-26 (2001)

Shimomachi, T.、N.K.Okamura、T.Takemasa 和 T.Takakura.：“通过微波传感对番茄植株水分胁迫进行无损检测。”Environ.Control in Biol.. 39. 17-26 (2001)