Environmental Control in Biological Engineering

生物工程环境控制

• 批准号: 62440018
• 负责人: TAKAKURA Tadashi
• 金额: $ 11.78万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62440018/
• 关键词: Biotechnology; Masspropagation; Tissue culture; Enbryogenic cells; 環境調節; 組織培養; バイオテクノロジー; ナーサリィーテクノロジー

Automatic screening system for enbryogenic cells and tissue culture vessels by means of ultrasonic misting have been developed in the present research year.Through the stage of callus, carrot somatic embryos were developed and they were screened through two kinds of meshes. the flow of liquid media was switched in different ways in order to prevent blocking of meshes.One experiment consisted of four continuous trials. Although not many cells can be obtained in the first two experiments, in the third trial the ratio of cells in the torpedo stage to the total cells over 300 micron meter was 163/683. It is concluded that the ratio of normal cells is relatively high and the blocking of meshes is not serious.Cymbidium PLB and potatoes were used for misting culture. It was found that there is no difference between the fresh weight of Cymbidium PLB in misting culture and that of liquid culture and absorption of sugar and water can be controlled by misting system although the dry weight in misting system is inferior.In potato cultivation, all vegetable growth in misting system is superior to the other.It would be necessary to solve high humidity condition in vessels as well as lower light intensity caused by water droplets on the vessel surfaces although the absorption of water and sugar is easily controlled by misting intervals.

本研究开发了基于超声波雾化技术的胡萝卜胚性细胞和组织培养容器的自动筛选系统。通过愈伤组织培养阶段，获得胡萝卜体细胞胚胎，并通过两种筛网进行筛选。以不同的方式切换液体介质的流量，以防止介质堵塞。一个实验由四个连续的实验组成。虽然前两次实验获得的细胞不多，但在第三次实验中，鱼雷阶段的细胞与300微米以上的细胞总数之比为163/683。结果表明，正常细胞率较高，滤网堵塞不严重。用大花兰和马铃薯混合培养。结果表明，喷雾培养的大花兰鲜重与液体培养的鲜重没有差异，虽然喷雾培养的干重较低，但通过喷雾系统可以控制糖和水的吸收；在马铃薯栽培中，所有蔬菜在喷雾系统中的生长都优于其他系统。尽管喷雾间隔很容易控制水分和糖的吸收，但需要解决容器内高湿度和容器表面水滴导致的光强较低的问题。

项目成果

Honjo,T.: "Effect of CO_2 enrichment on the growth of CYMBIDIUM PLB in vitro" Acta Horticulturae. 230. 185-188 (1988)

Honjo,T.：“CO_2 富集对蕙兰 PLB 体外生长的影响”《园艺学报》。

孫禎翼: "ジャ-ファ-メンタ培養におけるCO_2施用が培地のpHに及ぼす影響" 農業気象. 45. 1-5 (1989)

孙廷玉：“CO_2施用对Japharmenta培养中培养基pH的影响”农业气象45。1-5(1989)。

蔵田憲次: "超音波ミストを利用した組織培養装置の試作" 日本農業気象学会講演要旨. 18-19 (1989)

Kenji Kurata：“使用超声波雾的组织培养装置的原型生产”日本农业气象学会讲座摘要18-19（1989）。

孫禎翼: "植物工場におけるサラダ菜の蒸散量とチップバ-ンに対する培養液の電気伝導度と光条件の影響" 農業気象. 44. 253-258 (1989)

孙廷玉：“培养基电导率和光照条件对植物工厂沙拉蔬菜蒸腾和碎烧的影响”农业气象44。253-258（1989）。

後藤英司: "葉菜類の人工光栽培における光強度と照射コストの関係" 農業気象. 43. 229-232 (1987)

Eiji Goto：“叶类蔬菜人工光栽培中光强度与照射成本的关系”《农业天气》43. 229-232 (1987)。