Development of Environmentally safe Insect Regulators from Microbials

对苏云金芽孢杆菌以色列变种、aizawai变种和其他品种的杀虫蛋白对双翅目和鳞翅目昆虫的杀虫活性进行了评价。采用生物化学和基因工程方法对筛选出的杀虫蛋白及其基因进行分析。结果表明，昆虫幼虫将杀虫蛋白注入肠道，在肠道中加工产生活性片段，活性片段与肠道中的受体发生反应，停止进食。然后，在大肠杆菌、酵母、藻类和烟草植物中产生了活性片段和全蛋白，为昆虫防治开辟了新的途径。

The insecticidal proteins from Bacillus thuringiensis var.israelensis, var.aizawai, and other varieties were evaluated for insecticidal activity against diptera and lepidoptera insects.The selected insecticidal proteins and their genes were analyzed by biochemical and genetic engineering methods. It was revealed that insecticidal proteins were injested by insect larvae and processed in midguts to yield active fragments, which reacted with receptors in midguts to stop eating.Then, the active fragments and the whole proteins were produced in E.coli, yeast, algae and tobacco plants to develop novel approaches for insect control.