Use of Biosurfactants for Monitoring and Remediation of Persistent Organic Pollutants

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It was attempted to develop convenien polychlorinated biphenyls(PCBs) based on molecular mechanisms of biological functions in mammals. (1) Recombinant aryl hydrocarbon receptor(AhR)-mediatedβ-glucuronidase(GUS) reporter gene expression systems were each introduced into tobacco t biochemical assays of and Arabidopsis plants. The transgenic plants were developed for GUS assays of PCBs. Particularly, the use of biosurfactants improved GUS assay sensitivity of PCBs in the transgenic plants. (2) Monoclonal and recombinant antibodies specific to PCBs were prepared for development of Enzyme-Linked Immunosorbent Assays(ELISAs) of PCBs. The ELISAs selectively detected coplanar PCBs.