Generation of transgenic tobacco plants with enhanced drug-metabolism coupled with photosynthesis
Generation of transgenic tobacco plants with enhanced drug-metabolism coupled with photosynthesis
批准号:
07456030
负责人:
OHKAWA Hideo
金额:
$4.1万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1997
中文摘要
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英文摘要
The transgenic tobacco plants expressing rat P4501A1 and its fused enzyme with yeast P450 reductase in chloroplants were generated by genetic engineering. P4501A1 and its fused enzyme were coupled with photosynthetic electron transport chains in the chloroplants to exhibite an enhanced monooxygenase activity under light irradiation. The transgenic tobacco plants metabolized the herbicide chlorotoluron through N-demethylation and m-methyl hydroxylation to exhibite herbicide resistance. Particularly, m-methyl hydroxylation seemed to be important for herbicide resistance.
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M.Hara et.al.: "Regeneration of NADPH by Cactus Chloroplasts : Coupling Reaction with P450 Monooxygenase" Journal of Fermentation and Bioengineering. 84. 324-329 (1997)
M.Hara 等人:“仙人掌叶绿体再生 NADPH:与 P450 单加氧酶的偶联反应”发酵与生物工程杂志。
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通讯作者:
Young-Sug Kim et.al.: "Photo-induced Activation of Cytochrome P450/Reductase Fusion Enzyme Coupled with Spinach Chloroplasts" Biotechnology Techniques. 10. 717-720 (1996)
Young-Sug Kim 等人:“细胞色素 P450/还原酶融合酶与菠菜叶绿体的光诱导激活”生物技术。
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通讯作者:
M.Hara, H.Ohkawa, M.Narato, M.Shiraki, Y.Asada, I.Karube and J.Miyake: "Regeneration of NADPH by Cactus Chloroplasts : Coupling Reaction with P450 Monooxygenase" Journal of Fermentation and Bioengineering. 84. 324-329 (1997)
M.Hara、H.Ohkawa、M.Narato、M.Shiraki、Y.Asada、I.Karube 和 J.Miyake:“仙人掌叶绿体再生 NADPH:与 P450 单加氧酶的偶联反应”发酵与生物工程杂志。
DOI:
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H.Ohkawa et.al.: "Molecular Mechanisms of Herbicide Resistance" Reviews in Toxicology. 1. 1-8 (1997)
H.Ohkawa 等人:“除草剂抗性的分子机制”毒理学评论。
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通讯作者:
Y-Sug Kim, M.Hara, K.Ikebukuro, J.Miyake, H.Ohkawa and I.Karube: "Photo-induced Activation of Cytochrome P450/Reductase Fusion Enzyme Coupled with Spinach Chloroplasts" Biotechnology Techniques. 10. 717-720 (1996)
Y-Sug Kim、M.Hara、K.Ikebukuro、J.Miyake、H.Ohkawa 和 I.Karube:“细胞色素 P450/还原酶融合酶与菠菜叶绿体的光诱导激活”生物技术。
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共 9 条
Use of Biosurfactants for Monitoring and Remediation of Persistent Organic Pollutants
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批准号:21510095
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.0万
-
财政年份:2009
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负责人:OHKAWA Hideo
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依托单位:
Research and Development of Biomonitoring Technology for Environmental Health Chemicals
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批准号:11793012
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项目类别:Grant-in-Aid for University and Society Collaboration
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资助金额:$6.46万
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财政年份:1999
-
负责人:OHKAWA Hideo
-
依托单位:
Plant xenobiotic-metabolizing enzyme genes for phytoremeditation of environmental endocrine disruptors
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批准号:11460046
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.47万
-
财政年份:1999
-
负责人:OHKAWA Hideo
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依托单位:
Development of Biomonitoring Technology for Environmental Endocrine Disruptors
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批准号:11556016
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.08万
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财政年份:1999
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负责人:OHKAWA Hideo
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依托单位:
Development of Environmentally safe Insect Regulators from Microbials
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批准号:07556024
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$4.93万
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财政年份:1995
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负责人:OHKAWA Hideo
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依托单位:
国内基金
海外基金
CIA1 (Chloroplast Import Apparatus 1)调控拟南芥营养生长阶段转变的分子机理
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批准号:31300997
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项目类别:青年科学基金项目
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资助金额:22.0万元
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批准年份:2013
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负责人:李小冬
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依托单位: