The animal experimental study to examine the availability of fetus surgery in CL/Fr mouse feutses affected with cleft lip and palate

目的：胎鼠和胎鼠创面愈合的研究较少。本研究旨在探讨CL/Fr小鼠唇裂小鼠唇裂手术缝合后的胎儿愈合是否能在没有明显炎症反应的情况下发生，并伴有胶原沉积。材料和方法：本研究选用上唇裂贯通伤模型，术后对动物进行72小时的跟踪观察。取10~15周龄已交配的成年雌性CL/Fr小鼠，分别于妊娠第15天、第16天或第17天用Enthoren麻醉。每只动物都进行了肾切除术，以进入怀孕的子宫。每个实验胎儿都通过小子宫切开术和在距中线约2 mm的上嘴唇形成的穿透和穿透的外科伤口进行部分外切术。每个胎儿都被送回子宫内腔，子宫切开术以一个钱包st…结束。更多的环状缝合。剖腹手术伤口层层闭合，动物从麻醉中恢复。然后继续怀孕，直到胎儿被收获。受伤的胎儿被分成以下三组，每组包括10或11只动物。第一组，缝合正常胎儿嘴唇上的伤口。第二组胎儿唇裂仅缝合无创口，第三组采用11/0尼龙和显微外科技术缝合唇裂创面。每组分别于伤后24小时、48小时、72小时取胎10~11枚。每只胎儿在生理盐水中固定，制作苏木精-伊红染色的上唇水平切片。结果：通过上唇伤口的胎儿手术实验，成功获得胎鼠32只。每组伤后72小时取4~5个胎儿进行组织学检查。结果如下：1)第一组4个胎儿中有3个胎儿的两个创口间充质元素相互连接；2)第二组只有1个胎儿的两个创口间充质元素相互连接；3)第三组5个胎儿中有2个胎儿的两个创口间充质元素相互连接。这些结果提示CL/FR小鼠胚胎唇裂的两个切开部位间充质组织有可能再生。较少

Purpose : There is a deficiency of a study of wound healing in fetal mice or rats. The present study has been undertaken to examine whether or not fetal healing on surgical suture of clefted lip in CL/Fr mouse fetus affected with cleft lip can occur without an apparent inflammatory response and take place with the deposition of collagen.Material and Method : The experimental model chosen for this study was a through wound of the upper lip, and the animals were followed for 72 hours after surgery. The-mated adult female CL/Fr mouse of ten to fifteen weeks old were anesthetized with enthoren on the 15th day, 16th or 17th day of gestation. A laporotomy was performed in each animal to gain access to the pregnant uterus. Each experimental fetus was partially exteriorized through a small hysterotomy and a through and through surgical wound made in the upper lip approximately 2 mm from the mid line. Each fetus was returned to the lumen of the uterus, and the hysterotomy closed with a purse st … More ring suture. The laparotomy wounds were closed in layrs and the animals allowed to recover from the anesthetic. The pregnancies then continued nntil the fetuses were harvested. The injured fetuses were divided into the following three groups, each containing ten or eleven animals. In the first group, the wounds made on the normal lip of the unaffected fetuses were sutured. In the second group, the clefted lip of the affected fetuses were only sutured wothout wound, while in the third group, the wounds made on the clefted lip of the affected fetuses were sutured using 11/0 nylon and microsurgical techniques. In each group, ten or eleven fetuses were harvested at 24 hours, 48 hours or 72 hours after wounding. Each harvested fetus was fixed in formal saline, and haematoxylin and eosin stained horizontal sections of the upper lip were prepared.Result : A total of 32 fetal mice could be successfully obtained through the fetus surgery experiment of a wound of the upper lip. In each group, four or five fetuses harvested at 72 hours after wounding were subjected to a histological examination. The following results were obtained.1) The mesenchymal elements were connecting each other from both wounded sites in three out of four fetuses for the first group.2) The mesenchymal elements were connecting each other from both wounded sites in only one out of five fetuses for the second group.3) The mesenchymal elements were connecting each other from both wounded sites in two out of five fetuses for the third group.These results suggested the useful possibility for the mesenchymal tissue to regenerate from both incised site of the clefted lip in CL/Fr mouse fetuses. Less