Enhance of bone-implant interface using membrane techinique

引导骨再生（GBR）技术在骨增强术中的临床应用已经讨论了近十年。在这项研究中，我们使用膜技术研究了磷灰石种植体周围的新骨形成。在雄性新西兰大白兔胫骨内植入磷灰石2片植入物，然后覆盖可吸收膜（GC膜，GC,Tokyo, Japan）。实验组分为两组。其中一组（EL组）将胶原凝胶（bone Ject, Fujisawa, Tokyo, Japan）连接的真骨陶瓷置于骨表面与膜之间，另一组（E2组）不放置真骨陶瓷。对照组不应用膜陶瓷和真骨陶瓷。动物在2周、4周、8周后被处死。制备含种植体和周围组织的标本进行组织学检查。结果表明：1)与对照组相比，E1组应用膜获得的间隙几乎被新骨填充得更快。2) E2组应用膜获得的空隙中新骨填充速度较E1组慢。然而，在八周后，获得的空隙几乎被新骨填满。3) 8周后，膜基本保持原状，但有少许破坏。4)使用真正的骨陶瓷延缓了新骨的形成，然而，这些是有效的空间制造。

Clinical application of bone augmentation using the guided bone regeneration (GBR) techinique has been discused for last decade. In this study, we investigated new bone formation around the apatite implant uising a membrane technique. Apatite 2-Piece implants were inserted in tibiae of male New Zealand white rabbits and then covered with resorbable membranes (GC membrane, GC,Tokyo, Japan). Experimental groups were divided into two groups. In one group (EL groups), true bone ceramics connected by collagen gel (Bone Ject, Fujisawa, Tokyo, Japan) were placed between bone surface and membrane, In the other groups (E2 groups), true bone ceramics were not placed. In control groups, neither membrane or true bone ceramics was applied. Animals were sacrificed after two, four eight weeks. The specimens containing the implant and peripheral tissue were prepared for the histological examination. The results were the following :1) In E1 groups, the spaces aquired by application of the membranes were almost filled with new bone faster than those in control groups.2) In E2 groups, the spaces aquired by application of the membranes were filled with new bone slower than those in E1 groups. On eight weeks later, however, the spaces aquired were almost filled with new bone.3) On eight weeks later, mebranes kept their shape with a little destruction.4) New bone formation delayd by the use of true bone ceramics, however, those were effective for space making.