Studies on Sex Pheromones and Attractants of Geometrid Moths and Their Application.

(1)日本巨环蛾天然信息素的立体化学及合成信息素的田间试验日本巨环蛾Ascotis selenaria白垩纪Butler（鳞翅目：尺蛾科）是茶园的严重剥叶虫。GC- ms分析证实了(3Z,6Z,9Z) -壬二atriene和顺式-3,4-环氧化物的存在，GC和HPLC手性柱分析表明环氧化物为外消旋混合物。结果表明，纯(3R,4S) -同分异构体比其他对映异构体的活性更强，而(3S,4R) -同分异构体在以色列对亚种更有吸引力。这一结果表明，这些亚种不仅在分布上存在着生殖隔离，而且在信息素交流上也存在着生殖隔离。(2)巨环虫信息素光学活性类似物的随机筛选试验。用手性高效液相色谱柱分离了几种环氧二烯的外消旋混合物，并在混交林中测试了对映体的吸引活性。在1996年的试验中，三种没有被任何外消旋混合物吸引的几何物种，被用外相活性环氧化物作诱饵的陷阱捕获。此外，其他三种被外消旋混合物吸引的物种被光学活性环氧化物捕获的数量更多，这表明光学纯环氧化物的现场试验对研究几何信息素是有用的。

(1) Stereochemisty of the natural pheromone of the Japanese giant looper moth and field test of the synthetic pheromoneThe Japanese giant looper, Ascotis selenaria cretacea Butler (Lepidoptera : Geometridae), is a serious defoliator of a tea garden. GC-MS analysis of the virgin female extract confirmed the occurrence of (3Z,6Z,9Z) -nonadecatriene and the cis-3,4-epoxide, and further GC and HPLC analysis with chiral columns showed that the epoxide was a racemic mixture. The field evaluation for the male moths interestingly revealed stronger activity of the pure (3R,4S) -isomer than any other enantiomeric mixture, while the (3S,4R) -isomer attracted the sub-species in Israel. This result indicates that these sub-species are reproductively isolated not only by their distribution but also by the pheromonal communication.(2) Random screening tests with optical active analogs of the giant looper pheromone.Racemic mixtures of several epoxydienes were resolved with a chiral HPLC column, and attractive activities of the enantiomers were tested in a mixed forest. By the test in 1996, three geometrid species, which had not been attracted to any racemic mixtures, were newly captured by the traps baited with the eptical active epoxide. Besides, the other three species, which had been attracted to the racemic mixture, were captured more abundantly by the optical active epoxides, indicating usefulness of the field test with optical pure epoxides for the study of geometrid pheromones.