Is uric acid just a waste product? -Its role in antioxidation system in insects-

1.建立了一种高灵敏度的昆虫脂质过氧化物检测系统。该系统采用高效液相色谱和化学发光检测器（CL-HPLC）。过氧化甘油三酯的检出限为1 pmol。在某虫体样品中发现了未知的化学发光抑制剂，但经Sep-pak NH_2.2预处理后能有效去除。2 .用含有红素B的食物喂养洋葱蝇（Delia antiqua）幼虫，在日光灯照射下使其处于氧化应激状态，与不含红素B的对照昆虫相比，其含有更多的过氧化脂质。用家蚕研究了尿酸对氧化应激的作用。将黄嘌呤脱氢酶抑制剂别嘌呤醇添加到家蚕的人工饲料中，家蚕体内的尿酸含量降低到不可检测的水平。在紫外光照射下，贫尿酸家蚕的寿命明显短于正常家蚕。被尿酸耗尽的蚕也表现出对一种光毒性物质孟加拉玫瑰产生的氧化应激的抵抗力下降。

1.A highly-sensitive detection system for peroxides in a lipid fraction from an insect was developed. The system employs a high performance liquid chromatography and a chemiluminescence detector (CL-HPLC). The detection limit for triglyceride peroxide was 1 pmol. Samples from an insect body were found to contain unknown inhibitor(s) for chemiluminescence, but it was efficiently removed by a pretreatment with Sep-pak NH_2.2.The larvae of the onion fly, Delia antiqua was fed with a diet containing a phototoxicant, Erythrosine B.When the larvae was given an oxidative stress by illuminating them with a fluorescent lamp, the larvae contained a larger amount of lipid peroxide compared with the control insects (without Erythrosine B).3.The role of uric acid against the oxidative stress was examined using the silkworm, Bombyx mori. When an inhibitor of xanthine dehydrogenase, allopurinol, was added to the artificial diet of B.mori, the amount of uric acid in the body of B.mori grown on this diet was decreased to a nondetectable level. Under illumination with ultraviolet light, the silkworms depleted with uric acid showed a significantly shorter life span than the normal insects. The silkworms depleted with uric acid also showed decreased resistance against oxidative stress produced by a phototoxicant, rose bengal.