ldentification and detection of algicidal filamentous bacteria by DNA probe method

本研究采用DNA探针法对海洋环境中分离的杀藻丝状细菌进行了快速鉴定。试验菌株于1990 ~ 1995年从鹿儿岛湾海水、海藻和海洋植物中分离得到。得到了以下结果：检出菌株的GC%在34.6% ~ 38.9%之间，推测菌株属Saprospira属、Microscilla属或flexibacter属。1990年、1991年和1992年分离的菌株在同一年分离的菌株中检测到DNA-DNA杂交，而在不同年份分离的菌株中未检测到DNA-DNA杂交。3 . SS92-11与SS93-7之间、SS95-1与1990年分离的菌株之间存在DNA-DNA杂交，但SS95-4与其他菌株之间没有DNA-DNA杂交。根据16S rDNA PCR产物的RFLP模式，将试验菌株分为90年组、91年和95年组、92年和93年组。这些结果表明，DNA探针法是一种快速鉴定杀藻丝状菌的方法。

In this study, DNA probe method was investigated for rapid identification of algicidal filamentous bacteria isolated from marine environments. Test strains were isolated from sea water, marine algae and marine plants in Kagoshima Bay from 1990 to 1995. The following results were obtained.1. As GC% of test strains were found to be between 34.6 to 38.9%, they were suggested as to belong to the genera Saprospira, Microscilla or Flexibacter.2. For the strains isolated in 1990,1991 and 1992, DNA-DNA hybridization was detedted among the strains isolated in the same year while it was not among the strains in the different years.3. DNA-DNA hybridization was detected between SS92-11 and SS93-7 and among SS95-1 and the strains isolated in 1990, but it was not among SS95-4 and all other strains.4. On the basis of RFLP patterns for PCR products of 16S rDNA,test strains were classified into three groups such as 90 year group, 91 year and 95 year group, and 92 year and 93 year group.These results indicate that DNA probe method is useful for rapid identification of algicidal filamentous bacteria.