Method of Collection and Preservation of Urine Samples for Measurement of Urinary Volatile Organic Solvents as Indices of Biological Monitoring for Exposure to Organic Solvents

本研究的目的是开发一种有效的收集和保存尿液样本的方法，用于测量尿液高挥发性有机溶剂。因此，为了保存尿液样本，发现装有装满尿液的样品瓶（样品瓶中没有顶部空间）的冷库是非常可用的。加入水溶性β -环糊精和羟丙基衍生物（2- hp - β - cd）的尿样更易保存。另一方面，对于混合有机溶剂，建立了多组分标准溶液的同时简易制备方法，用于毛细管柱气相色谱法测定尿液中有机溶剂。即，2- hp - β - cd同时加标制备多组分标准溶液，稳定性好。通过对有机溶剂使用车间的调查，有机溶剂暴露浓度与尿中有机溶剂暴露浓度呈显著相关：相关系数(r)和回复线方程（y=ax）分别为甲苯[r=0.950, y=0.0270x]、MIBK [r=0.900, y=0.352x]、MEK [r=0.919, y=0.519x]、IPA [r=0.940, y=1.095x]。由有机溶剂暴露浓度与尿液有机溶剂暴露浓度的回归线方程，可计算出暴露阈值所对应的尿液有机溶剂浓度。在尿液有机溶剂浓度的情况下，未校正的观察值与有机溶剂暴露浓度的相关性比校正值（通过肌酐或比重）更显著。本研究建立了尿液挥发性有机溶剂测定的尿液样本采集保存方法，发现有机溶剂暴露浓度与尿液有机浓度存在显著相关性。少

This study was for the purpose of developing an available method of collection and preservation of urine samples for measurement of urinary highly volatile organic solvents.Consequently, for preservation of urine samples, it was found out that the cold storage with a sample vial full of urine (there was no head space in the sample vial) was very available. And urine samples spiked with water-soluble beta-cyclodextrin and hydroxypropyl derivative (2-HP-beta-CD) was more available for preservation.On the other hand, for exposure to mixed organic solvents, the simultaneous simple preparation method of a multi-component standard solution was established for the measurement of urinary organic solvents using gaschromatography with capillary column. Namely, a simultaneous multi-component standard solution spiked with 2-HP-beta-CD was easily prepared with stability.From the investigation in the workshop using organic solvents, the relation between the concentration of organic solvent exposure … More and that of urinary organic solvent showed a significant correlation : correlation coefficient (r) and equation of regression line (y=ax) were toluene [r=0.950, y=0.0270x], MIBK [r=0.900, y=0.352x], MEK [r=0.919 y=0.519x], IPA [r=0.940, y=1.095x]. From the equation of the regression line between the concentration of organic solvent exposure and that of the urinary organic solvent, urinary organic solvent concentrations corresponding with exposure to threshold limit value could be calculated.In the case of concentration of urinary organic solvents, the observed value that was uncorrected showed more significant correlation with the concentration of organic solvent exposure than the corrected values (by creatinine or specific gravity).This study established the method of collection and preservation of urine samples for measurement of urinary volatile organic solvents, and showed significant correlation between the concentration of organic solvent exposure and that of urinary organic concentration. Less