Bone formation by the implantation of bone marrow cells

在本研究中，我们试图通过植入从骨髓基质细胞分化而来的成骨细胞来形成大量的骨。目前已有地塞米松或维生素C用于诱导成骨细胞分化，但尚未见此类细胞在体内成骨的报道。最近，我们发现胶原凝胶在不含地塞米松或维生素C的情况下诱导成骨细胞分化，这些细胞在体内诱导骨和骨体积呈时间依赖性增加。另一方面，未分化的骨髓细胞在胶原胶中不能形成骨。这些结果表明，表达成骨细胞表型的骨髓基质细胞可以在体内有效地成骨。胶原凝胶对I型胶原有特异性作用，而对II型-V型胶原无明显作用。具有诱导活性。此外，转化生长因子-β通过胶原凝胶促进成骨细胞的分化。我们的结果表明，通过我们的程序，骨髓细胞可以形成一团骨。我们方法的好处是1)我们不使用地塞米松，这有很强的副作用，2)我们使用的是胶原，它与组织有亲和力。

In this study, we attempted to form a mass of bone by the implantation of osteoblastic cells differentiated from bone marrow stromal cells. Dexamethasone or vitamin C have been utilized to induce the osteoblastic differentiation so far, however, there is no report that these cells formed bone in vivo. Recently we found that collagen gel induced the osteoblastic differentiation without dexamethasone or vitamin C.These cells with collagen gel induced bone and a volume of bone increased time-dependently in vivo. On the other hand, un-differentiated bone marrow cells with collagen gel did not form bone. These results indicate that bone marrow stromal cells which expressed the osteoblastic phenotypes could form bone effectively in vivo. The effect of collagen gel is specific for type I,and type II-V collagen did no? possess the inducing activity. Furthermore, TGF-beta enhanced the osteoblastic differentiation by collagen gel. Our results demonstrate that bone marrow cells could form a mass of bone by our procedure. The benefit of our method is 1) we do not use dexamethasone, which has a potent side-effect, 2) we utilized collagen, which shows affinity to tissues.