Microsatellite instability and oncogene expression in gastric cancer

22.4%的胃癌组织中有Cyclin E过表达。这些指标与肿瘤的侵袭深度和淋巴结转移显著相关。Cyclin E过度表达患者的生存率明显低于无Cyclin E过度表达患者，c-met和c-erbB-2过度表达的病例分别为46.1%和16.4%。C-met和c-erbB-2基因扩增分别在10.2%和11.7%的胃癌组织中检出。C-met的扩增和过度表达与肿瘤的侵袭深度和淋巴结转移显著相关，c-erbB-2的扩增和过度表达与组织分化程度显著相关。C-met或c-erb B-2基因扩增和/或过度表达患者的生存率明显低于未扩增或过度表达患者。我们检测了42例胃癌手术标本中2号和3号染色体上三个微卫星标记基因座的DNA遗传不稳定性。微卫星不稳定性与临床病理特征无关。

Overexpression of cyclin E was obserbed in 22.4% of gastric cancer cases. Those were significantly corraleted with depth of invasion and lymph node metastasis. The survival rate of the patients with cyclin E overexpression was significantly poorer than that of the patients without cyclin E overexpression.Overexpression of c-met and c-erbB-2 was obserbed in 46.1% cases and 16.4% cases, respectively. Gene amplification of c-met and c-erbB-2 was detected in 10.2% and 11.7% of gastric cancer cases, respectively. Amplification and overexpression of c-met was significantly correlated with depth of invasion and lymph node metastasis, while amplification and overexpression of c-erbB-2 was significantly correlated with histologic differentiation. The survival rate of the patients with c-met or c-erbB-2 amplification and/or overexpression was significantly poorer than that of the patients without amplification or overexpression.We examined DNA from 42 surgical specimens of gastric cancers for genetic instability at three microsatellite marker loci on chromosomes 2 and 3. We detected differences between tumor and normal DNA banding patterns in 12 of the 42 tumors examined (28.6%). There was no correlation between microsatellite instability and clinicopathological features.