Specific expression of molecules in the tissues of cholesteatoma otitis media -3'prime cDNA subtraction method analysis-

1.提取胆脂瘤型中耳炎(CHOLE)、慢性化脓性中耳炎(OMC)及外耳道皮肤(皮肤)组织中的信使ENAs(MRNAs)，并将其逆转录成cDNA。2.3‘端片段文库消减实验表明，肝细胞生长因子(HGF)及其受体c-MET在Chole-cDNA文库中表达上调，且其拷贝数显著高于OMC和皮肤cDNAs文库。3.差异显示结果还表明，核孔复合体中Tpr(易位启动子区域)基因的mRNA表达水平显著高于OMC和皮肤cDNA文库。HGF/c-Met系统在包括角质形成细胞在内的多种上皮和非上皮细胞的增殖过程中可能起重要作用。尽管Tpr基因的潜在功能尚未确定，但Tpr蛋白被认为是一种具有转化活性的磷酸化蛋白。本研究提示HGF/c-Met系统和Tpr基因都可能参与胆脂瘤性中耳炎角质形成细胞的增殖和肉芽组织的形成。

1.Messenger ENAs (mRNAs) in tissues of cholesteatoma otitis media (Chole), chronic purulent otitis media (OMC), and skin of external ear canal (Skin) were extracted and reverse-transcribed into the cDNAs. These cDNAs were compared and analyzed by using the 3' prime cDNA library subtraction method and the differential display method.2.The 3' prime cDNA library subtraction indicated that the expressions of mRNAs of hepatocyte growth factor (HGF)and c-met, the specific HGF receptor, were up-regulated in the Chole-cDNA library, and that the copy numbers for these cDNAs were extremely higher in this library than those in the OMC and skin-cDNAs libraries.3.The differential display also demonstrated that the mRNA expression of Tpr (translocated promoter region) gene, a component of the nuclear pore complex, was also up-regulated in the Chole-cDNAs.4.It has been suggested that HGF/c-met system should play an important role in poliferation of several epithelial and non-epithelial cells including keratinocyte. Although potential functions of Tpr gene have not been determined, Tpr protein has been suggested to be a phosphoprotein and have a transforming activity.The present study suggests that both HGF/c-met system and Tpr gene could be involved in proliferation of keratinocytes and development of granulation tissues observed in cholesteatoma otitis media.