Cytotoxicity testing of dental materials with saliva chemical factors in a culture medium

为了检验含有唾液因子的培养液的细胞可持续性，用两种人工唾液(绿木、唾液)和人唾液培养细胞(HEp-2、L-929、RC2)，然后在新鲜的培养液中回收细胞。通过将人工唾液添加到45%的培养液中，大约70%的细胞被回收。用Hep-2、Gin-1两种细胞株和加入45%人工唾液和人唾液的组织模型(MATREX)检测了8种金属和3种聚合物假体材料的细胞毒性。钛、银钯合金和热固性树脂无细胞毒性。钴铬合金和锡银合金表现出轻微的细胞毒性。银铟合金、镍铬合金和镍铜镓合金具有中等的细胞毒作用。铜和两种自固化树脂效果显著。这两种细胞系之间没有发现差异。目前的发现表明，唾液/组织模型对用于牙科材料的细胞毒性测试可能是有用的，尽管这种组合并不完全支持细胞培养。

To examine the cell sustainable composition of a culture medium containing saliva factors, two kinds of artificial saliva (Greenwood, Salivate) and human saliva were used to cultivate cells (HEp-2, L-929, RC2), followed by cell recovery in a fresh medium. By adding artificial saliva to a medium at 45%, approximately seventy percent of the cells were recovered. Cytotoxicities of eight metallic and three polymeric prosthetic materials were then examined by the cell recovery rate using two cell lines (HEp-2, Gin-1) and tissue model (MATREX) with addition of artificial saliva and human saliva at 45% to medium in vitro. Titanium, silver palladium alloy and heat-cured resin were not cytotoxic. A cobalt chromium alloy, and tin silver alloy showed only slight cytotoxicity. Silver indium alloy, nickel chromium alloy and nickel copper gallium alloy had moderate cytotoxic effects. Copper and two self-curing resins had marked effects. No difference between the two cell lines was found. The present findings suggest that saliva / tissue model couples for cytotoxicity testing of dental materials may be useful, despite the fact that the combination did not fully support cellular cultivation.