Antioxidative substance in sea weeds and the activity to inhibit oxidative damage

我们研究了海藻对活性氧化剂的结合活性。海藻用搅拌机粉碎，然后用氯仿和甲醇提取。用开柱将提取物分为极性或非极性化合物，并用溶剂分离。采用黄嘌呤/黄嘌呤氧化酶体系化学发光法测定抑制活性氧化剂的活性。采用TBA法和红细胞膜胶泥体系测定其抑制脂质过氧化的活性。在筛选试验中，部分极性或非极性部分的化合物表现出抑制活性氧化剂的活性。进一步用高效液相色谱仪对活性物质进行了分离，深绿色组分具有较强的抗氧化活性，并对其进行了分离纯化。将分离得到的化合物定名为叶绿酸a，比较了叶绿酸a和β-胡萝卜素抑制活性氧化剂的活性对人体健康的贡献。

We investigated the binding activity of seaweeds against active oxidant. Seaweeds were powdered by a blender, and extracted with chloroform and methanol. The extracts were classified into the polar or nonpolar compounds with the solvents by a open column. The activity of extracts inhibited active oxidant were measured by chemiluminesence method with xanthine/xanthine oxidase system. The activity inhibited lipid peroxidation were by TBA method with the erythrocyte membrane goust system.In the screening test, some compounds in the polar or nonpolar section showed the activity inhibited active oxidant. Further, the active compounds were fractionated by HPLC.Dark green fraction showed the strong antioxidative activity, and the fraction was separated and purified. The compound was isolated as pheophytin a. Pheophytin a was compared with beta-carotene about the activity inhibited active oxidant in the point of the contribution to the human health and body.