Preparations of Cast Film of Aligned DNA Doule Srtands

我们通过混合来自布道睾丸的DNA水溶液和阳离子脂质分子来制备DNA-脂质复合物。沉淀的DNA-脂质复合物可溶于大多数有机介质，但不溶于水溶液。当DNA-脂质复合物的氯仿溶液流延在玻璃板上时，获得透明的、物理上坚固的膜。结果表明：1）将铸膜单向拉伸，得到了DNA取向膜，并通过X射线衍射分析证实了这一点; 2）将DNA-脂质铸膜浸泡在溴化乙锭水溶液中，由于溴化乙锭染料分子的插入，膜被染成红色。薄膜的偏振吸收光谱证实了染料分子的取向。3）DNA取向的流延膜表现出很大的电导率各向异性。观察到静电导电性沿着膜中的DNA链，但不垂直于DNA链。

We have prepared DNA-lipid complex by mixing both aqueous solutions of DNA from sermon testes and cationic lipid molecules. The precipitated DNA-lipid complex is soluble in most of organic media, bur not in aqueous solutions. When chloroform solution of DNA-lipid complex was cast on a glass plate, a transparent, physically strong film was obtained. Following results were obtained.1)A cast film was stretched in one direction, the DNA-aligned film was obtained, which was confirmed by X-rya diffraction analyses.2)When the DNA-lipid cast film was soaked in aqueous solution of ethidium bromide, the film was colored to red due to the intercalated ethidium bromide dye molecules. Orientation of intercalated dye molecules could be confirmed by polarized absorption spectra of the film.3)DNA-aligned cast film showed a large anisotropic behavior of conductivity. The electrostatic conductivity was observed along the DNA strands in the film, but not perpendicular to the DNA strands.