Molecular mechanisms of the membrane disorder in the crush syndrome patients by the Kobe Earthquake

1.研究了4例阪神大地震挤压综合征患者和健康人红细胞膜超长链脂肪酸的分布。用气相色谱-质谱法(GC-MS)鉴定了顺式-17-己二烯酸(C26：1)和十六烷酸(C26：0)。C26：1/C22：0、C26：0/C22：0、C24：1/C22：0和C20：1/C22：0比值升高。这些发现表明，挤压综合征中缺血再灌注产生的活性氧物种和/或自由基导致了过氧化酶体中的β-氧化障碍。我们研究了10例健康对照和29例神户地震伤员血浆中一氧化氮的变化。硝酸盐还原为亚硝酸盐后，用高效液相色谱-紫外检测法同时测定亚硝酸根(NO2-)和硝酸根(NO3-)的含量。摄入精氨酸或饮食衍生亚硝酸盐对健康志愿者的血浆亚硝酸盐和硝酸盐含量无影响。挤压综合征患者血浆硝酸盐含量升高，而肾功能正常的患者血浆亚硝酸盐和硝酸盐含量均不增加。这一发现提示挤压综合征患者一氧化氮合成增加和/或一氧化氮排泄减少。

1. Profiles of very long-chain fatty acids were studied in the erythrocyte membrane of healthy controls and four patients with crush syndrome caused by the Great-Hanshin Earthquake. The identities of cis-17-hexacosenoic acid (C26 : 1) and hexacosanoic acid (C26 : 0) were verified by gas chromatography-mass spectrometry (GC-MS). The ratio C26 : 1/C22 : 0, C26 : 0/C22 : 0, C24 : 1/C22 : 0.and C20 : 1/C22 : 0 also were elevated. These findings suggest that active oxygen species and/or free radicals generated by ischemia-reperfusion in the crush syndrome result in beta -oxidation disorders in peroxisomes.2. We investigated the nitric oxide profile in the plasma of 10 healthy controls and 29 patients hurt by the Kobe Earthquake. The levels of nitrite (NO_2^-) and nitrate (NO_3^-) were measured simultaneously by high-performance liquid chromatography (HPLC) with UV detection using the Griess reaction after the reduction of nitrate to nitrite. Arginine consumed in food or diet-derived nitrite had no effect on the plasma nitrite and nitrate contents of the healthy volunteers.Plasma nitrate was elevated in the crush syndrome patients ; whereas neither nitrite nor nitrate was increased in patients with normal renal function. This finding suggests increased nitric oxide synthesis and/or decreased excretion of nitric oxide in the crush syndrome.