Application of gene therapy to acute lung injury

（1）人类研究：我们用溴化胺的劳动积累了芦荟宏观。聚合宏观的部分是确定的。然后我们可以测量噬细胞凋亡和微生物活性。我们还利用了聚合酶链反应（PCR）的力量来增强AMs噬菌体和微生物学活动中的前炎症细胞因子的mRNAs，随着时间的推移，在异氟醚中毒期间，去酶的发生率大于异丙酚中毒。Gene expression in IL-1、IL-8、IFN-D2、TNF-D2、D2 increased over time。您的位置：知道173>> IL-8 and IFN D2 were greater in isoflurane than in propofol anesthesia。我们报告了在焦虑和手术期间对脉冲免疫学功能的渐进性影响。我们的数据认为，脉搏缺陷是由焦虑症的类型调制的，就像焦虑症和外科的持续时间一样好。（2）动物研究：老鼠被机械化地感染了哈洛酮、恩氟酮、异氟酮或七氟氟酮。在整个脉冲杆菌之后，我们研究了亲炎症细胞因子的基因表达。Gene expression for IL-1，MIP-2， IFN-D2 γ-D2， and TNF γ-D2 all increased significantly compared with Mechanical ventilation alone。这些数据索引是指在机械通风的基础上，在炎症反应下对挥发性病理学进行了描述。

(1) human research : We harvested alveolar macrophages by bronchoalveolar lavage over time. The fraction of aggregated macrophages was determined. We then measured phagocytosis and microbicidal activity. We also exploited the power of the polymerase chain reaction (PCR) to amplify mRNAs of pro-inflammatory cytokines from AMs Phagocytic and microbicidal activity progressively decreased over time, with the decrease being greater during isoflurane than propofol anesthesia. Gene expression in IL-1, IL-8, IFNィイD2γィエD2, TNFィイD2αィエD2 increased over time. The increases in IL-8 and IFNィイD2γィエD2 were greater in isoflurane than in propofol anesthesia. We report progressive impairment of pulmonary immunologic function during anesthesia and surgery. Our data suggest that pulmonary defenses are modulated by the type of anesthesia, as well as the duration of anesthesia and surgery.(2) Animal study : Rats were mechanically ventilated with halothane, enflurane, isoflurane, or sevoflurane. After whole pulmonary lavage, we examined gene expression of the pro-inflammatory cytokines. Gene expression for IL-1, MIP-2, IFNィイD2γィエD2, and TNFィイD2αィエD2 all increased significantly compared with mechanical ventilation alone. These data indicate that inhalation of volatile anesthetics under mechanical ventilation induces an inflammatory response.