Isolation of human motile sperm without chromosomal error, and induction of acrosome reacti

咖啡因（cafe）刺激精子活力和顶体反应（AR），这种作用被认为是通过抑制磷酸二酯酶和导致cAMP的积累而出现的。本研究考察了钙通道阻滞剂对AR的影响，认为钙对运动性和AR的作用机制不同。环AMP可刺激运动性，而Caf对AR的作用是通过刺激钙内流而不是cAMP积累来实现的。本研究发现人类精浆中存在腺苷。腺苷受体激动剂PIA刺激AR，拮抗剂8-SPT抑制AR，提示精子表面腺苷受体参与AR诱导的可能性，精子不能在精浆中诱导AR，而精浆的禁欲是自发诱导AR的触发因素，本研究结果提出了精浆中腺苷是精浆中抑制AR的工作假设。而咖啡碱的作用是作为腺苷受体的拮抗剂出现的。

Caffeine (Caf) Stimulates both sperm motility and acrosome reaction (AR), and the action is considered to be appeared through inhibition of phosphodiesterase and resulting accumulation of cAMP.The present study examined the effect of calcium channel blocker on AR,and concluded that the action mechanisms of Caf on motillity and AR were different each other. Motility was stimulated by cyclic AMP,in contrast the action of Caf on AR was appeared through the stimulation of calcium influx, but not cAMP accumulation.The present study discovered the presence of adenosine in human seminal plasma. Adenosine receptor agoist, PIA,stimulated AR and the antagosist, 8-SPT,suppressed AR.This facts suggested the posibility that sperm surface adenosine receptor contribute to the induction of AR.The sperm can not induce AR in the seminal plasma, and the abstinence form the seminal plasama is the trigger of spontaneous induction of AR.The present results proposed the working hypothesis that adenosine in the seminal plasma was the repressing factor in the seminal plsama, and the action of Caf was appeared through as the antagonist against the adenosine receptor.