Study of the regulation mechanism of ciliogenesis

1.研究了人输卵管上皮纤毛发生的超微结构。在基体和纤毛之间的过渡区形成了短管状结构，称为过渡管。细管直径34- 36nm，长度0.13 (]SY.+- . [)0.06 nm，在纤毛发生过程中短暂存在，被认为在纤毛伸长过程中固定基体和/或在翼片形成中起重要作用。纤维状颗粒与条纹细根的形成密切相关。原始的根被分成两个不同的部分，与基部相连的近圆锥形部分和远纤维状部分。由于纤维状部分在成熟纤毛器中不明显，这部分被认为是在纤毛发生过程中退化或变成了圆锥形部分。通过对大鼠脱落细胞顶皮层注射产生的单克隆抗体R4109的鉴定，发现了一种新的与斑纹根相关的蛋白。R4109在免疫印迹上识别了一个58 kDa的蛋白，在免疫荧光和免疫电镜上识别了有纹路的根。R4109在输卵管和呼吸道纤毛细胞、室管膜细胞和视网膜感光细胞中有纹状小根。与单生纤毛相关的条纹细根也被染色。R4109在PtK2和HF等培养细胞中显示出条状根，但未形成单生纤毛。免疫组化观察纤毛细胞R4109修饰纤维颗粒，提示纤维颗粒与纤毛形成有关。

1.I investigated the ultrastructure of, ciligenesis in the human oviduct epithelium. Short tubular structures, termed transitinal tubules, were formed in the transitional zone between the basal body and cilium. The tubules, 34-36 nm in diameter and 0.13 (]SY.+-。[)0.06mum in length, existed transiently during ciliogenesis and were considered, to fix the basal body during ciliary elongation and/or play an important role in alar sheet formation.Fibrous granules were deeply concerned with the formation of striated rootlets. Primitive rootlets were divided into two distinct parts, the proximal conical partconnected to the basal body and the distal fibrillar part. Since the fibrillar part was obscure in the mature ciliary apparatus, this part was considered as being eitherdegraded or changed into the conical part during ciliogenesis.2.A novel protein associated with striated rootlets were found by identifying a monoclonal antibody R4109 which was produced by injecting to apical cortices of deciliated cells to rats. R4109 recognized a 58 kDa protein on immunoblotting and decorated striated rootlets on immunofluorescence and immunoelectron microscopy. R4109 decorated striated rootlets in ciliated cells of the oviduct and respiratory tract, ependymal cells, and retinal photoreceptor cells.Striated rootlets associated with the solitary cilium were also stained. Striated rootlets were demonstrated by R4109 in culture cells such as PtK2 and HF without formation of the solitary cilium. Fibrous granules were decorated by R4109 in immunohistochemical investigation of ciliogenic cells, suggesting strongly that fibrous granules were concerned with rootlet formation.