Endocrine cell tumors of gastrointestinal tract : molecular biological study of their histogenesis progression, and metastasis

在过去的两年中，本项目分析了约400例日本人胃肠道内分泌细胞肿瘤（GECT）和6个内分泌细胞癌细胞系.产品分类：结果：1.将GECT分为两大类癌（CD）低度恶性组和内分泌细胞癌（ECC）高度恶性组，建立了GECT的组织学鉴别诊断和新的发生部位分类.组织发生：CD主要来源于未成熟的内分泌细胞。ECC可能来源于粘膜管状腺癌/腺瘤中的肿瘤性内分泌细胞、全能干细胞、CD或未成熟的内分泌细胞。第一种途径被怀疑是最常见的，其次是第二种。CD和ECC中p53蛋白表达的差异证实了两者组织发生途径的不同.进展和转移：GECT的细胞增殖活性被证实与肿瘤进展和转移密切相关。对于临床激素分析评估肿瘤复发和转移，GECT应细分为三组; argentaffin（产生降钙素）CD，argyphi 1（产生肽）CD和ECC（经常产生降钙素）。细胞系：从人食管、胃和直肠ECC建立的四种细胞系良好地保存了激素（5-羟色胺、肽YY、降钙素）的产生。一种食管细胞系受曲马多菌素和丝裂霉素的影响。

During the period of past two years of this project, about 400 gastrointestinal endocrine cell tumors (GECT) of Japanese and six cell lines of endocrine cell carcinoma were analyzed.1. CLASSIFICATION : GECT were divided into two main groups : carcinoid tumor (CD), low-grade malignancy and endocrine cell carcinoma (ECC), high-grade malignacy.Histological differential diagnosis and the new classification according to ocurring sites of GECT were established.2. HISTOGENESIS : CD was suspected to be mainly derived from immature endocrine cell. ECC was hypothesized to derive from neoplastic endocrine cell in mucosal tubular adenocarcinoma/adenoma, totipotential stem cell, CD,or immature endocrine cell. The first pathway was suspected to be most frequent, followed by the second. Difference in p53 protein overexpression between CD and ECC confirmed the difference in their histogenetic pathway.3. PROGRESSION AND METASTASIS : Cell proliferation activity of GECT was confirmed to correlate well with tumor progression and metastasis. For clinical hormone analysis to assess tumor recurrence and metastasis, GECT should be subdivided into three groups ; argentaffin (serotonin-producing) CD,argyrophi1 (peptide-producing) CD,and ECC (frequently serotonin-producing).4. CELL LINES : Four cell lines established from human esophageal, gastric and rectal ECC well preserved hormone (serotonin, peptide YY,calcitonin)-production. One esophageal cell line was effected by Vincristin and Mitomycin.