Establishment of hypertansive congenic Strains

我们一直在以正常血压对照Wistar-Kyoto大鼠（WKY）为背景，开发具有卒中易发自发性高血压大鼠（SHRSP）高血压基因的同源大鼠品系。1)遗传标记的制备(1)通过与小鼠和人类基因组的比较，我们选择了几个可能位于大鼠1号染色体上高血压相关区域的基因。我们从相应大鼠基因的基因组克隆中分离出11个遗传标记。b)采用随机扩增微卫星多态性（RAMP）方法（Wu et al., 1994）获得了100多个SHRSP与WKY之间多态的共显性标记。2)同源菌株的构建将SHRSP与WKY进行5代回交，得到半同源菌株（95%以上的基因组来自WKY）。用微卫星标记进行基因分型，筛选各代回交大鼠的基因型。目前已获得6株在大鼠1号染色体上具有不同区域的半同源菌株。我们现在检查这些菌株的血压，并进一步进行回交，以制造完整的同源菌株。

We have been developing congenic rat strains that possess the hypertension genes of stroke-prone spontaneously hypertensive rats (SHRSP) on the background of a normotensive control, Wistar-Kyoto rats (WKY).1) Preparation of genetic markersa) We selected several genes probably located in the hypertension-related region on rat chromosome 1by comparing rat genome with mouse and human genomes. We isolated 11 genetic markers from the genomic clones of the corresponding rat genes.b) We applied the randomly amplified microsatellite polymorphisms (RAMP) method (Wu et al., 1994) to get more than 100 codominant markers that are polymorphic between SHRSP and WKY.2) Construction of the congenic strainsSHRSP were sequencially backcrossed to WKY for 5 generations to get the semi-congenic strains (more than 95% of genome were from WKY). Genotypes of the backcross rats of each generation were screened by genotyping of microsatellite markers. We have so far obtained 6 semi-congenic strains that have different regions on rat chomosome 1.We now check blood pressure of these strains as well as backcross them further to make complete congenic strains.