Investigation of the usefulness of pentoxifylline for liver preservation

我们研究了连续低温灌注保存（CHPP）和戊茶碱（PTX）联合应用在无心跳供体（NHBD）肝移植中的作用。三个实验构成了这项研究。(1)我们从NHBD进行了猪肝移植。在本实验中，我们使用采购后的CHPP，并将PTX放入保存液中。因此，我们得出结论，CHPP与PTX是一个有用的程序，以改善从NHBD移植肝的功能。(2)本研究的目的是确定PTX预处理是否可以影响一氧化氮（NO）的释放，并在大鼠肝脏热缺血损伤有有益的影响。因此，PTX预处理增加了热缺血期间NO的产生。PTX对大鼠肝脏热缺血损伤有保护作用，NO对热缺血损伤有抑制作用。(3)本研究采用CHPP法保存大鼠肝脏，并探讨其最佳的灌流液。因此，UW-葡萄糖酸盐溶液被认为适用于CHPP，因为它抑制蛋白水解并保护肝细胞。

We have investigaed that the usefulness of a combination of continuous hypothermic perfusion preservation (CHPP) and pentoxifylline (PTX) for liver transplantation from non-heart beating donors (NHBD). Three experiments constituted this study. (1) We performed porcine liver transplantation from NHBD. In this experiment, we used CHPP after procurement and put PTX in preservation solution. As a result, we concluded that CHPP with PTX is a useful procedure to improve the function of liver grafts from NHBD. (2) The purpose of this study is to determine whether PTX pretreatment could influence nitric oxide (NO) release and have a beneficial effect on warm ischemic injury in rat liver. As a result, PTX pretreatment increase NO production during warm ischemia. And PTX prevent warm ischemic injury and NO inhibit warm ischemic injury in rat liver. (3) In this study, we preserved rat livers by CHPP, and investigated what solution was most suitable as the perfusate. As a result, the UW-gluconate solution was considered suitable for CHPP, because it suppressed proteolysis and protected liver cells.