Study on molecular architecture and its application

在细胞表面，许多受体蛋白被提呈，这些受体蛋白识别特定的生物分子。这些机理可以从化学角度进行分析。为了使生物目标系统化，对这些生物分子的分子结构进行了研究。Matsunaga博士使用细菌磁性颗粒(BMP)研究了分子结构。利用锚定蛋白MAGA或新分离的BMP膜蛋白MMS16，通过基因融合，成功地在BMP上展示了荧光素酶、蛋白A和受体等功能蛋白。蛋白A显示的BMPs用于胰岛素的化学发光免疫分析和高灵敏度分析。此外，雌激素结合实验也证实了BMPs上活性受体的表达。开发了一种利用天然蛋白MAGA将功能蛋白固定在BMP上的新方法，即体外直接插入法。通过这种技术，活性荧光素酶可以成功地展示在BMP上。…Kobatake博士进一步研究了位点特异性脂质标记抗2-苯基恶唑酮单链抗体的展示方法，并通过基因工程制备了用于在脂质体表面展示抗体分子的脂质标记抗体结合蛋白。脂质标记蛋白在大肠杆菌中表达，并在细菌膜上转位。通过将脂尾锚定到脂体膜上的疏水层，将它们稳定地整合到高取向的脂质体中。小宫山博士研究了β-环糊精(β-CyD)与二异氰酸酯连接物的交联以及分子印迹。结果表明，分子印迹效应在调节宿主分子协同结合客体分子方面是有效的。后藤健二博士研究了脂质体表面的结构。该表面活性剂-酶复合体的反应速率是天然酶的100倍以上。研究了含有新合成糖脂的脂质体的生物靶向性。通过结合试验评价脂质体的靶向作用程度，发现糖脂具有良好的生物靶向作用。较少

In cell surface, many recepter proteins are presented and the specific biomolecules are recognized by these recepter proteins. These mechanisms can be analyzed from chemical view points. To systemize the biotergeting, the molecular architecture of these biomolecules were investigated. Dr. Matsunaga studied molecular architecture using bacterial magnetic particles (BMPs). Successful display of functional proteins such as luciferase, proteinA and receptor on BMP was demonstrated using anchor proteins, MagA or a newly isolated BMP membrane protein Mms16, through gene fusion. ProteinA-displayed BMPs was used for chemiluminescence immunoassay of insulin and high sensitive assay. Furthermore, expression of active receptor on BMPs for estrogen binding assay was also confirmed. A novel approach, in vitro direct insertion, for immobilizing functional proteins on BMPs using the native protein, MagA was developed. Through this technique, active luciferase could be successfully displayed on BMPs. … More Dr. Kobatake studied the displaying method using site-specific lipid-tagged anti-2-phenyl -oxazolone single-chain antibody and lipid-tagged antibody-binding protein were prepared by genetic engineering for displaying antibody molecules on liposome surfaces. The lipid-tagged proteins were expressed in E. coli and translocated at the bacterial membrane. They were stably incorporated into liposomes with high orientation by anchoring the lipid-tail into the hydrophobia layer on the liposome membrane. Dr. Komiyama studied the cross-linkage of β-cyclodextrin (β-CyD) with diisocyante linker and molecular imprinting to the molecule. It was suggested that the molecular imprinting effect is effective in regulating host molecules to cooperatively bind guest molecules. Dr.Goto studied the architecture on the liposome surface. The reaction rate of the surfactant-protease complex was 100 times higher than that of the native one. A biotargeting effect of liposomes that includes the newly synthesized sugar lipid was studied. The degree of targeting effect of liposome was evaluated by the binding test and it was found that the sugar lipid exhibited an excellent bio-targeting effect. Less