Molecular analysis of UV-A resistant operon in a marine cyanobacterium.

海洋蓝细菌中 UV-A 抗性操纵子的分子分析。

基本信息

项目摘要

A filamentous cyanobacterium Oscillatoria NKBG091600 can grow under UV-A irradiation with white light. The cyanobacterium produces biopterin-glycoside, UV-A absorbing compound, in the cell depending on the UV-A irradiation. In this report, molecular mechanisms of the UV-A tolerant growth of the cyanobacterium were studied. Under the growth of UV-A radiation, a protein of 60 kDa was highly expressed at 8 hours after irradiation. The expression of this protein was accompanied by an increase of biopterin-glucoside amount. N-terminal amino acid sequence of the 60 kDa protein was homologous to cyanobacterial GroEL which was known well as a heat shock protein in various organisms. Northern hybridization analysis showed that the level of groEL mRNA increased depending on the UV-A irradiation, showing that the induction of GroEL was due to the activation of transcription level. A genomic DNA fragment containing the GroESL operon (GroES+GroEL) in the cyanobacterium was cloned by using PCR with … More primers designed from those reported from cyanobacteria. By a detailed analysis of the gene structure, CIRCE (Controlled Inverted Repeat of Chaperone Expression) region was found at upper stream of GroES, while the distance between the CIRCE and GroES were two-fold longer than those in Synechococcus PCC 7942 and Synechocystis PCC 6803. A SOS box-like region was also found between the CIRCE and GroES.It was not found in Synechococcus PCC 7942 and Synechocystis PCC 6803. The SOS box-like region functioned as SOS regulator in E.coli cells. These differences in genome structure indicated that expression of GroESL iut the UV-A tolerant cyanobacterium was regulated by complex way comparing from those in other cyanobacteria. In addition to the increase of biopterin-glycosides and GroEL, myxoxanthophyll was also found to increase in the UV-A tolerant cyanobacterium. UV-A tolerance of the cyanobacterium resulted on complex mechanism containing at least three factors, (1) induction of biopterin-glycoside production, (2) induction of GroESL transcription, and (3) accumulation of carotenids and xanthophylls. Future studies will be required for developing the techniques for creation of UV-A tolerant organisms. Less
丝状蓝藻颤藻NKBG 091600能在白色光的UV-A辐射下生长。蓝细菌在细胞中产生生物蝶呤糖苷,即UV-A吸收化合物,这取决于UV-A照射。本文对蓝藻耐UV-A生长的分子机制进行了研究。在UV-A辐射的生长下,在辐射后8小时高表达60 kDa的蛋白。该蛋白的表达伴随着生物喋呤-葡萄糖苷量的增加。N-末端氨基酸序列的60 kDa的蛋白是同源的蓝藻GroEL,这是众所周知的热休克蛋白在各种生物。北方杂交分析表明,groEL mRNA的表达量随UV-A辐射的增强而增加,表明GroEL的诱导是由于转录水平的激活。利用PCR技术克隆了蓝细菌中含有GroESL操纵子(GroES+GroEL)的基因组DNA片段, ...更多信息 根据报道的蓝藻引物设计引物。通过对基因结构的详细分析,发现CIRCE(Controlled Inverted Repeat of Chaperone Expression)区域位于GroES的上游,且CIRCE与GroES之间的距离是Synechococcus PCC 7942和Synechocystis PCC 6803的两倍。在CIRCE和GroES之间还发现了一个SOS盒状区域,而在聚球藻PCC 7942和集胞藻PCC 6803中没有发现。SOS盒样区域在大肠杆菌细胞中起SOS调节剂的作用。这些基因组结构上的差异表明,与其他蓝藻相比,耐UV-A蓝藻中GroESL的表达受到复杂的调控。除了生物蝶呤糖苷和GroEL的增加,还发现在UV-A耐受性蓝藻中粘叶黄素增加。蓝细菌的UV-A耐受性导致至少包含三个因素的复杂机制,(1)诱导生物喋呤-糖苷产生,(2)诱导GroESL转录,和(3)积累类胡萝卜素和叶黄素。今后需要开展研究,以开发创造耐紫外线A生物体的技术。少

项目成果

期刊论文数量(54)
专著数量(0)
科研奖励数量(0)
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专利数量(0)
Tadashi Matsunaga: "Micro-aerobic Hydrogen Production of Photosynthetic Bacterium Rhodovulum sp. by a Double Phase Photobioreactor."Biotech. Bioeng.. (in press).
Tadashi Matsunaga:“通过双相光生物反应器对光合细菌红酵母进行微需氧产氢。”生物技术。
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Haruko Takeyama: "Analysis of Salinity-Regulated Replication of an Endogenous Plasmid pSY10 from the Marine Cyanobacterium Synechococcus sp."Appl. Biochem. Biotechnol.. (in press).
Haruko Takeyama:“来自海洋蓝藻聚球藻的内源质粒 pSY10 的盐度调节复制分析”。
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Mitsufumi Matsumoto: "Screening of marine cyanobacterial buoyancy for application of environmental monitoring."J.Appl.Phycol.. (in press).
Mitsufumi Matsumoto:“用于环境监测应用的海洋蓝藻浮力筛选。”J.Appl.Phycol..(出版中)。
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竹山春子: "生物を利用したCO2のリサイクルと地球環境問題"日刊工業新聞. 12 (1999)
Haruko Takeyama:“利用生物体回收二氧化碳和全球环境问题”日刊工业新闻 12 (1999)。
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Tadashi Matsunaga: "Micro-aerobic Hydrogen Production of Photosynthetic Bacterium Rhodovulum sp.by a Double Phase Photobioreactor."Biotech.Bioeng.. 68. 647-651 (2000)
Tadashi Matsunaga:“通过双相光生物反应器进行光合细菌Rhodovulum sp.的微需氧产氢。”Biotech.Bioeng.. 68. 647-651 (2000)
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MATSUNAGA Tadashi其他文献

MATSUNAGA Tadashi的其他文献

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{{ truncateString('MATSUNAGA Tadashi', 18)}}的其他基金

Development of functional nano-particles using biomineralization process in magnetic bacteria
利用磁性细菌生物矿化过程开发功能性纳米颗粒
  • 批准号:
    20246119
  • 财政年份:
    2008
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Proteome Analysis of Magnetite Crystal Formation Related Proteins Based on Genomic Information
基于基因组信息的磁铁矿晶体形成相关蛋白的蛋白质组分析
  • 批准号:
    18206084
  • 财政年份:
    2006
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
ANALYSIS OF NGF SIGNAL IN NEUROBLASTOMA AND ITS CLINICAL APPLICATION
神经母细胞瘤NGF信号分析及其临床应用
  • 批准号:
    13671865
  • 财政年份:
    2001
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of Mechanisms for Biomagnetite Formation and Its Application
生物磁铁矿形成机制的阐明及其应用
  • 批准号:
    13002005
  • 财政年份:
    2001
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
Development of miniaturized fully-automated immunoassay robot using magnetic particles from recombinant magnetic bacteria
利用重组磁性细菌磁性颗粒开发小型化全自动免疫分析机器人
  • 批准号:
    10555285
  • 财政年份:
    1998
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on molecular architecture and its application
分子结构研究及其应用
  • 批准号:
    10145102
  • 财政年份:
    1998
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas (A)
Development of simplified electrochemical allergy diagnostic system.
开发简化的电化学过敏诊断系统。
  • 批准号:
    05555229
  • 财政年份:
    1993
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Modify of the bacterial magnetic particles by gene manipulation
通过基因操作修饰细菌磁性颗粒
  • 批准号:
    05453109
  • 财政年份:
    1993
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Analysis of genes concerned with bacterial magnetite production
与细菌磁铁矿生产相关的基因分析
  • 批准号:
    03453128
  • 财政年份:
    1991
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Leucocyte Separation Using Magnetotactic Bacteria
使用趋磁细菌分离白细胞
  • 批准号:
    63850190
  • 财政年份:
    1988
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B).

相似海外基金

Nitrogen limitation and synthesis of UV absorbing compounds and domoic acid in the toxic alga Pseudo-nitzschia multiseries
有毒藻类拟菱形藻中的氮限制以及紫外线吸收化合物和软骨藻酸的合成
  • 批准号:
    443789-2013
  • 财政年份:
    2013
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Understanding the biosynthesis of marine UV-absorbing compounds
了解海洋紫外线吸收化合物的生物合成
  • 批准号:
    DE120101412
  • 财政年份:
    2012
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Discovery Early Career Researcher Award
Physiological Ecology of UV-Absorbing Compounds in Antarctic Organisms
南极生物中紫外线吸收化合物的生理生态学
  • 批准号:
    9017664
  • 财政年份:
    1991
  • 资助金额:
    $ 7.81万
  • 项目类别:
    Continuing Grant
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