Development of simplified electrochemical allergy diagnostic system.

开发简化的电化学过敏诊断系统。

• 批准号: 05555229
• 负责人: MATSUNAGA Tadashi
• 金额: $ 12.22万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05555229/
• 关键词: allergy; allergen; IgE antibody; blood; chemical mediator; biosensing; 食物アレルギー; 白血球; サイクリックボルタメトリー; メディエーター

Many immunoassay methods have been developed for sensing or detecting allergens. The skin tests including prick test and scratch test are typical methods. However, the these tests are dangerous because of anaphylaxis by serum antibodies. Therefore, a safe, simple and rapid method for detecting allergic reaction is required in the clinical and food industry fields. We have investigated the electrochemical method of detection of foods which cause an allergic reaction. A sample containing protein fraction of food was added to cells and incubated. When the potential was applied in the range 0-1.0V vs.saturated calomel electrode, an anodic peak current appeared at around 0.3V.This peak current, attributed to serotonin, increased with time, and the maximum current was obtained 20-25 min of allergen addition. Then, a relationship between applied potential and current difference of whole blood with and without serotonin was examined using whole blood and interdigitated array electrode. The current difference was maximized around 0.35V.The current increased linearly with the increasing concentration of serotonin in the range of 50 to 800 ng/ml. The response of the RBL-1 cells was specific to protein fraction of wheat.

已经开发了许多免疫测定方法用于感测或检测过敏原。皮肤试验包括点刺试验和划痕试验是典型的方法。然而，这些测试是危险的，因为过敏反应的血清抗体。因此，在临床和食品工业领域需要一种安全、简便、快速的过敏反应检测方法。我们研究了检测引起过敏反应的食物的电化学方法。将含有食物的蛋白质部分的样品加入细胞中并孵育。在0 ~ 1.0V（相对于饱和甘汞电极）的电位范围内，在0.3V左右出现一个由5-羟色胺引起的阳极峰电流，该峰电流随时间的延长而增大，在加入变应原20-25 min时达到最大值。然后，使用全血和交叉指状阵列电极来检查具有和不具有5-羟色胺的全血的施加电位和电流差之间的关系。在50 ~ 800 ng/ml范围内，电流随5-羟色胺浓度的增加而线性增加。RBL-1细胞对小麦蛋白质组分的反应是特异性的。

项目成果

Tae-Kyu Lim: "Luminescene immunoassay for allergen using alkalince phosphatase-conjugated IgE antibody with flow injection system" Denki Kagaku. 63. 1154-1159 (1995)

Tae-Kyu Lim：“使用碱性磷酸酶缀合的 IgE 抗体和流动注射系统对过敏原进行发光免疫测定”Denki Kagaku。

Tae-Kyu Lim, Noriyuki Nakamura and Tadashi Matsunaga: ""Luminescence immunoassay for allergen using alkaline phosphatase-conjugated IgE antibody with flow injection system."" Denki Kagaku. 63. 1154-1159 (1995)

Tae-Kyu Lim、Noriyuki Nakamura 和 Tadashi Matsunaga：“使用碱性磷酸酶缀合的 IgE 抗体和流动注射系统对过敏原进行发光免疫测定。”Denki Kagaku。

Tae-Kyu Lim: "Luminescence immunoassay for allergen using alkaline phosphatase-conjugated IgE antibody with flow injection system" Denki Kagaku. 63. 1154-1159 (1995)

Tae-Kyu Lim：“使用碱性磷酸酶缀合的 IgE 抗体和流动注射系统进行过敏原发光免疫测定”Denki Kagaku。

Noriyuki Nakamura, Shizuko Kumazawa, Koji Sode and Tadashi Matsunaga: ""Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells."" Sensors and Actuators B. 13/14. 312-314 (1993)

Noriyuki Nakamura、Shizuko Kumazawa、Koji Sode 和 Tadashi Matsunaga：“使用大鼠嗜碱性白血病 (RBL-1) 细胞开发过敏反应快速检测系统。”传感器和执行器 B. 13/14。

Noriyuki Nakamura, Shizuko Kumazawa and Tadashi Matsunaga: ""In vitro electrochemical detection of wheat allergen using rat basophilic leukaemia (RBL-1) cells."" Applied Microbiology and Biotechnology. 43. 622-625 (1995)

Noriyuki Nakamura、Shizuko Kumazawa 和 Tadashi Matsunaga：“使用大鼠嗜碱性白血病 (RBL-1) 细胞对小麦过敏原进行体外电化学检测。”应用微生物学和生物技术。