High-Performance Chiral Separation Systems Based on Polysaccharide Derivatives

1.合成了纤维素和直链淀粉的环己基氨基甲酸酯类手性固定相，并考察了它们对手性固定相的拆分能力。CSPs具有较高的拆分能力，也可用于薄层色谱。这些衍生物可溶于氯仿，并在~1H核磁共振波谱和HPLC.2中对某些手性化合物表现出手性识别。甲壳素是一种与纤维素和直链淀粉一样容易获得的1，4-糖苷键的多糖。然而，直到最近，这种多糖还没有成功地用于制备高效液相色谱用CSPs。我们找到了一种制备甲壳素氨基甲酸酯的合适方法，如3，5-二甲基、4-氯和4-三氟甲基苯基氨基甲酸酯，具有较高的手性识别能力。甲壳素3，5-二甲基氨基甲酸酯和3，5-二氯苯基氨基甲酸酯的…可以在氯仿存在的情况下使用由于洗脱液在洗脱液中的溶解性较差，洗脱剂较多。我们研究了胆汁酸及其衍生物晶体包合物中的分子识别。许多不对称碳和多个氢键基团的存在使我们能够检查其分子结构的任何点突变对手性识别的显著影响。从液态有机化合物的衍生物重结晶得到适合于单晶X射线分析的包裹体晶体。主体组件大多具有双层结构，该双层结构涉及带有一些凹槽的一维空间。本研究对分子识别的大小、形状、极性和手性进行了合理的解释。我们发现，含有L氨基酸寡肽残基并被L氨基酸衍生物印迹的膜优先识别L异构体而不是相应的D-异构体，反之亦然。用反映其吸附选择性的膜实现了对映体选择性电渗析。较少

1. Cyclohexylcarbamates of cellulose and amylose were prepared and their resolving abilities for enantiomers were evaluated as chiral stationary phases (CSPs) for high-performance liquid chromatography (HPLC). The CSPs showed high resolving abilities, and could also be used for thin-layer chromatography (TLC). These derivatives were soluble in chloroform and exhibited chiral discrimination to some chiral compounds in ^1H NMR spectroscopy as well as in HPLC.2. Chitin is a readily available polysaccharide with 1,4-glycosidic linkage as well as cellulose and amylose. However, until recently this polysaccharide had not successfully been used for the preparation of CSPs for HPLC. We have found a suitable method for the preparation of chitin carbamates, such as 3,5-dimethyl, 4-chloro and 4-trifluoromethylphenylcarbamates, with relatively high chiral recognition abilities. The CSPs of chitin 3,5-dimethyl and 3,5-dichlorophenylcarbamates can be used in the presence of chloroform as a component … More of eluents owing to their poor solubilities in the eluent.3. We studied the molecular recognition in crystalline inclusion compounds of bile acids and their derivatives. The existence of many asymmetric carbons and multiple hydrogen bonding groups enabled us to check notable effects of any point mutations of their molecular structures on chiral recognition. Recrystallization of the derivatives from liquid organic compounds afforded inclusion crystals suitable for single-crystal X-ray analysis. The host assemblies mostly have bilayered structures which involve one-dimensional spaces with some pockets. This research led to the reasonable interpretation of molecular recognition in size, shape, polarity and chirality.4. We have found that a membrane which contains an oligopeptide residue from an L-amino acid and is imprinted by a L-amino acid derivative recognizes the L-isomer in preference to the corresponding D-isomer, and vice versa. Enantioselective electrodialysis was attained with the membranes reflecting their adsorption selectivity. Less