Nano-Chemistry in Liquid by Photothermal Spectroscopy

我们开发了几种分析方法，以澄清纳米空间中液相的化学现象，并在本项目中显示了它们的实用性。在高灵敏度的液体纳米空间分子检测中，对利用光热效应的热透镜显微镜进行了改进和优化。光热信号强度的估计更精确，以考虑样品的表面形态，因此我们成功地分析了细胞上的抗原-抗体反应。此外，为了将该方法应用于250μm宽通道内的螯合反应监测，我们可以首先将流动注射分析集成到玻璃芯片上。接下来，我们提出了一种可以监测液/液界面分子数的准弹性激光散射方法，并对分子转移和反应进行了原位研究。研究了不同表面活性剂在界面上的转移、相转移催化的界面行为以及水解酶对DPPC的水解等方面的新结果。利用飞秒脉冲激光建立了超快透镜效应方法，阐明了液体中溶质到溶剂的能量传递机理和溶质-溶剂效应，研究了光激发分子运动和反应。在Auramin O在低粘度溶液中的分子动力学中，发现了几个ps内的快速弛豫过程，并且弛豫时间与溶剂的平均分子量有关。在氨基偶氮苯光异构反应中，首次测定了中间态弛豫过程对溶剂的依赖性。激光击穿诱导等离子体的受激拉曼散射在极早期的几十秒内就被发射出来，这是液体纳米空间中的一种新现象。所得到的拉曼光谱具有一定的特征，并将其作为一种新的液体结构分析方法进行了研究。上述基于光热效应和毛细波的分析方法适用于非荧光分子，灵敏度与荧光方法相当。因此，可以断定，液体纳米化学的新世界是由它们打开的

We have developed several analytical methods in order to clarify chemical phenomena in nanospace in liquid phase and shown their usefulness in this project. In highly sensitive molecular- detection in liquid nanospace, the thermal lens microscope using photothermal effects was evolved and optimized for practical applications. The photothermal signal intensity was estimated more precisely to consider the surface morphology in samples, so that we succeeded in the analysis of the antigen-antibody reactions on the cell. Further, to apply this method to monitor the chelating reaction in the 250μm width channel, we could first integrate the flow injection analysis on the glass chip. Next, we have advanced the quasi-elastic laser scattering method which can monitor the number of molecules at liquid/liquid interface, and the molecular transfer and the reaction were studied in situ. New result have been obtained on the transfer of various surfactants at the interface, the interfacial behavior o … More f phase transfer catalysis, and the hydrolysis of DPPC by a hydrolysis enzyme.Ultra fast lens effect method using femtosecond pulse laser has been developed to clarify the energy transfer mechanism from solute to solvent in liquid and the solute-solvent effect, has studied in photo-excited molecular motion and reaction. In the molecular dynamics of Auramin O in low-viscosity solutions, the fast relaxation process within several ps was found out and it was found that the relaxation time depended on the mean molecular weight of solvent. In photo isomerization of amino azobenzen, the solvent dependence on the relaxation process in the intermediated state was firstly measured.It has been founded as the novel phenomena in the liquid nanospace that the stimulated Raman scattering in laser breakdown induced plasma was emitted in the very early stage within several tens seconds. The Raman spectra obtained were characteristic and we have investigated it as the new structural analysis method in liquid.The above-mentioned analytical methods, which are based on the photothermal effect and capillary wave, are applicable for non-fluorescent molecules and their sensitivity is comparable to the fluorescent methods. It is, therefore, concluded that new nano-chemistry world in liquid is opened by them Less