Identification of adipose-specific genes and teir clinical significance

过量体脂的积累已成为非胰岛素依赖型糖尿病（NIDDM）和动脉粥样硬化性血管疾病（AVD）发展的共同基础。体脂分布独立于上述疾病的发病风险。腹腔内内脏脂肪而非皮下脂肪的沉积与NIDDM和AVD的发生密切相关。本研究的目的是阐明皮下脂肪组织和内脏脂肪组织的生物学差异，并将数据应用于临床医学。通过比较皮下和内脏脂肪组织的基因表达谱，发现皮下脂肪主要表达维持基本细胞功能的蛋白质的“静态”基因，包括核糖体蛋白和细胞骨架蛋白。另一方面，内脏脂肪主要表达多种具有生物活性的分泌蛋白基因。我们重点研究了两个基因，即脂联素，这两个基因是通过…More对脂肪组织cDNA文库进行大规模随机测序获得的，以及通过差分显示技术分离的克隆085。脂联素是一种由脂肪组织特异性产生和分泌的血浆蛋白。然而，肥胖受试者的血浆脂联素水平下降，尤其是那些有内脏脂肪堆积的受试者。与bmi匹配的对照组相比，冠状动脉疾病患者血浆脂联素浓度明显降低。因此，血浆脂联素降低已被证明是冠状动脉疾病的潜在风险。该蛋白显示抗动脉粥样硬化特性，包括抑制内皮细胞的单核细胞粘附和血管疾病的增殖。克隆085的产物属于造血因子，在肥胖的发展过程中，内脏脂肪中克隆085 mRNA的表达增加。克隆085产品的血浆水平与小鼠内脏脂肪量平行增加。在人类中，血浆085水平与内脏脂肪的数量密切相关，但与皮下脂肪无关。在本研究中，我们探讨了内脏脂肪的来源因素。测量这些内脏脂肪衍生因子的血浆水平将为理解内脏脂肪肥胖伴随疾病的发病机制提供线索。少

Accumulation of excess body fat has become a common basis for the development of non-insulin dependent diabetes mellitus (NIDDM) and atherosclerotic vascular diseases (AVD). Body fat distribution is a independent r the risk for above diseases. Deposition of intra-abdominal viscreal fat rather than subcutaneous fat closely relates to the development of NIDDM and AVD. The aim of this study is to clarify the biological difference between subcutaneous and visceral fat tissues and to apply the data to clinical medicine.By the comparison of the gene expression profiles of subcutaneous and visceral fat tissues, subcutaneous fat has been revealed predominantly to express "static" genes for the proteins maintaining fundamental cellular functions including ribosomal protein and cytoskeleton proteins. On the other hand, visceral fat predominantly expressed the genes for a variety of secretory proteins with biological activities.We focused on two genes, that is, adiponectin, which was obtained by … More a large-scale-random sequencing of adipose-tissue cDNA library and clone 085, which was isolated by diferential display technique. Adiponectin is plasma protein specifically produced and secreted from adipose tisuue. Plasma levels of adiponectin, however, decreased in the obese subjects, especially those with visceral fat accumulation. Marked reduction of plasma adiponectin concentration was observed in he patients with coronary artey disease compaired with BMI-matched conrols. Therefore, decreased plasma adponectin has been proven to be a potential risk for coronary artery disease. The proein showed anti-atherogenic properties, including suppresion of monocyte-adhesion to endothelial cells and proliferation of vascular diseases.The product of clone 085 belonged to hematopoietic factors The expression of mRNA for clone 085 increased in visceral fat during the development of obesity. Plasma levels of clone 085 products increased in paralel with the amount of visceral fat in mice. In humans, plasma 085 level was closely correlated with the amount of visceral fat but not with subcutaneous fat.In this study, we investigated in the visceral fat derived factors. The measurement of plasma levels of these visceral fat derived factors will be a clue for understanding the pathogenesis of diseases accompanied by visceral fat obesity. Less