MALDI-TOF/TOF Mass spectrometer

MALDI质谱法是大型非极性分子的最佳电离方法，因此对乌尔姆大学化学研究所的几个研究小组至关重要。该仪器是1998年购买的，目前在质谱服务中心集中操作，利用率很高。在该仪器上进行的分析服务包括有机聚合物和大环、金属配合物、肽、聚糖和寡核苷酸的身份控制，从1D和2D凝胶带中鉴定蛋白质，以及通过“肽图谱”定位翻译后修饰。这些服务在用户条例的框架内提供给乌尔姆大学自然科学学院的所有研究小组。该仪器无法替代高质量范围（100 - 10,000 Da）的测量，具有高样品吞吐量的测量，并作为高分辨率FTICR仪器的“备用仪器”。然而，最近，由于仪器使用寿命长导致电子元件老化，停机时间和维修时间越来越多。制造商的支持在2014年停止，维修必须在内部进行，备件数量有限。由于设备停机时间长，功能有限，不再可能进行可靠和连续的服务操作。因此，兹要求提供财政支助，以一种新的更强大的MALDI-TOF/TOF仪器代替。

MALDI mass spectrometry is the optimal ionization method for large and nonpolar molecules and for this reason essential for several research groups of the Chemical Institutes of the University of Ulm. The instrument, which is currently centrally operated at the Mass Spectrometry Service Center and is very well utilized, was purchased in 1998. The analytical services performed on this instrument include identity control of organic polymers and macrocycles, metal complexes, peptides, glycans and oligonucleotides, identification of proteins from 1D and 2D gel bands and localization of post-translational modifications by "peptide mapping". These services are available to all research groups within the Faculty of Natural Sciences of the University of Ulm within the framework of the user regulations. The instrument is without alternative for measurements in the high mass range (> 10,000 Da), measurements with high sample throughput and as a "backup instrument" for the high-resolution FTICR instrument. Recently, however, downtime and repairs have been accumulating due to aged electronic components caused by the long lifetime of the instrument. Support from the manufacturer was discontinued in 2014, and repairs must be performed in-house with a limited number of spare parts still available. Due to high equipment downtime and limited functionality, reliable and continuous service operation is no longer possible. Therefore, financial support for a replacement by a new more powerful MALDI-TOF/TOF instrument is hereby requested.