Development of Drop-based Visual Test of Hemoglobin in Whole Blood and Its Application to Diagnosis of Anemia

已经发现，在十八烷基硅化（ODS）硅板上，一些染料溶质沿着水珠的周长集中。近年来，分散的微颗粒通过蒸发形成环的研究在理论和实验上引起了很大的兴趣。在本研究中，ODS板作为一个固体表面，形成一个不蒸发的环。罗丹明B荧光环用于全血血红蛋白目测。当一滴稀释过的血液和染料放在平板上时，在黑暗中用紫外线灯通过这滴血液观察到的荧光环只适用于血红蛋白含量低的样品。亲水蛋白，血红蛋白，不被吸附在平板上。其深红色通过水滴影响荧光环的激发和视觉感知。选择染料浓度是为了区分120 g/L和100 g/L血红蛋白之间的细微差异，这分别对应于健康和轻度贫血的女性受试者。在一个单独的实验中，发现三苯基甲烷染料在ODS板上的分配行为随三个苯环上取代基的变化而变化。这表明，环的形成将归因于所使用的染料的高疏水性。该方法的优点可应用于贫血的筛查试验。

It has been found that some dye solutes become concentrated along the perimeter of an aqueous drop on an octadecylsilanized (ODS) silica plate. There has been much theoretical and experimental interest recently in the formation of ring from dispersed micro-particles through evaporation. In this study, ODS plate serves as an solid surface to form a ring without evaporation. The fluorescent ring of Rhodamine B was applied to a visual test of hemoglobin in whole blood. When a drop of diluted blood and the dye is set on the plate, the resulting fluorescent ring can be observed through the drop with an ultraviolet lamp in the dark only for the samples of low hemoglobin content. The hydrophilic protein, hemoglobin, is not adsorbed on the plate. Its deep red color affects the excitation and visual perception of the fluorescent ring through the drop. The dye concentration was chosen to distinguish a slight difference between 120 and 100 g/L hemoglobin, which corresponds to healthy and slightly anemic female subjects, respectively.In a separate experiment, it was found that the partition behavior of the triphenylmethane dyes on an ODS plate varied with the substituted groups on the three phenyl rings. This indicates that the ring formation would be atributed to the high hydrophobicity of the dye used.The advantages of the proposed method can be applied to the screening test of anemia.