Study on roles of α and θ toxins in the pathogenesis of the acute renal failure in gas gangrene of Clostridium perfringens

α和θ毒素在急性肾衰竭发病中的作用，因为它是梭状芽孢杆菌气坏疽的危及生命的并发症之一。本研究得到的结果如下：1。通过左肾动脉注射32 HU θ毒素处理大鼠左肾，右肾不处理。术后第1天右肾菊粉清除率为0.925±0.012,4.165±0.509，左肾菊粉清除率为0.151±0.010,0.672±0.214。切除的左肾在体外灌注。2 .灌注液中的θ毒素（1HU/ml）引起灌注压突然升高。1 HU/ml θ毒素处理后肾动脉环对乙酰胆碱的松弛反应被抑制，30 HU/ml θ毒素处理后肾动脉环对乙酰胆碱的松弛反应消失，而毒素处理对苯肾上腺素的收缩反应影响不大。

Roles of α and θtoxins in the pathogenesis the acute renal failure because it is one of the life-threatening complications of the clostridial gas gangrene. The results obtained in this study are as follows :1. The left kidney of a rat was treated with 32 HU of θtoxin by injecting the toxin into the left renal artery and the right kidney was left untreated. Inulin and paminohippuric acid clearance measured one day after the operation were 0.925±0.012, 4.165±0.509 in the right kidney and 0.151±0.010, 0.672±0.214 in the left kidney, respectively.2. The left kidney excised out was perfused ex vivo. θ toxin (1HU/ml) in the perfusion fluid caused abrupt increase in the perfusion pressure.3. Relaxation response of renal arterial rings to acetylcholine was inhibited after the treatment of the rings with 1 HU/ml of θtoxin and disappeared by the treatment with 30 HU/ml of the toxin while contractile response to phenylephrine was little affected by the toxin treatment.