Interferon-induced proteins in chronic hepatitis.

干扰素诱导蛋白的表达和基因多态性与慢性病毒性肝炎的发病机制有关。对24例慢性丙型肝炎和10例慢性B型肝炎患者肝活检标本进行了PKR基因mRNA的检测。PKR的表达与肝炎病因、肝炎分期及IFN治疗反应无关。对12例慢性丙型肝炎患者肝活检组织中MxA基因mRNA进行了检测。MxA的表达与肝炎分期及IFN治疗反应无关。我们研究了MxA基因的变异是否能预测最近报道的IFN治疗持续应答（SR）的可能性。采用聚合酶链反应（PCR）限制性片段长度多态性（RFLP）技术检测MxA基因启动子区单核苷酸多态性（SNP）。对60例慢性丙型肝炎患者用干扰素治疗，观察其与干扰素疗效的关系。18例患者存在SR，其中31例MxA基因启动子区nt-88处G/G纯合子，SR率为32.3%。G/T杂合子17例，SR率23.5%。T/T同源异型12例，SR率33.3%。各基因型间SR率差异不显著。在47例基因型1感染者中，SR发生率G/G纯合子为20.8%，G/T杂合子为18.8%，T/T纯合子为14.3%。MxA基因表达与基因型无相关性。因此，我们的研究并没有证实IFN诱导蛋白的表达或基因多态性与慢性病毒性肝炎发病机制的相关性。

The expression and gene polymorphism of interferon (IFN)-induced proteins are suspected to correlate with pathogenesis of chronic viral hepatitis. mRNA of double-stranded RNA-dependent protein kinase (PKR) gene was measured in liver biopsy specimens of 24 patients with chronic hepatitis C and 10 patients with chronic hepatitis B. There found no correlation of expression of PKR with causes of hepatitis, stage of hepatitis or response to IFN therapy. mRNA of MxA gene was measured in biopsy specimens of 12 patients with chronic hepatitis C. There found no correlation of expression of MxA with stage of hepatitis or response to IFN therapy. We examined whether variation in MxA gene would predict the likelihood of sustained response (SR) to IFN therapy as recently reported. Single nucleotide polymorphism (SNP) in promoter region of MxA gene was determined by polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) technique. Its relationship to outcome of IFN therapy for chronic HCV infection was studied in 60 patients who were treated by IFN. 18 patients had SR. G/G homozygote at nt-88 of promoter of MxA gene was detected in 31 patients and SR rate was 32.3%. G/T heterozygote was detected in 17 patients and SR rate was 23.5%. T/T homo2ygote was detected in 12 patients and SR rate was 33.3%. There was no significant difference in SR rate among genotypes. In the 47 patients infected with genotype 1, SR rate did not significantly differ among genotypes : 20.8% in G/G homozygote, 18.8% in G/T heterozygote, and 14.3% in T/T homozygote. There was no correlation between expression of MxA gene and genotypes of MxA gene. Thus our study did not confirm the correlation of the expression or gene polymorpnism of IFN-induced proteins with pathogenesis of chronic viral hepatitis.