Analysis of biological response of epidemal components to simple chemicals

As is known in the case of Langerhans cells，dendritic cells play a crucial role in the initiation of immunity to simple chemicals such asnoted in the contact hypersensitivity.自DCs are scattered in非lymphoid organs as immaturecells.他们必须activated to initiate primary antigen specific immune reactions. Therefore，我们hypothesized that some simple chemicals must affect the function of DCs. In this paper，我们首先demonstrated human monocyte-derived DCs responded to such simple chemicals as DNCB，TNCB， DNFB, NiCl - D22 - D2, MnCl - D22 - D2, CoCl - D22 - D2, SnCl - D22 - D2D24 - D2 by augmenting their expression of CD86 or HLA-DR antigen，down-regulating c-Fms expression or increasing their production of TNFα。In addition，the DCs stimulated with the chemicals demonstrated increased allogeneic T细胞stimulatory function。接下来，我们发现，among these chemicals，only NiCl D22 - D2 and CoCl D22 - D2 induced apoptosis in them. Finally，我们examined the effects of these chemicals on CD86 expression by 3 different macrophage subsets andDCs induced from the cultures of human peripheral blood monocytes in the presence of M-CSFM-CSF + IL-4, GM-CSF + IL-4, respectively. Among them，only DCs dramatically augmented their expression of CD86.这些observations have revealed uniquecharacteristics of DCs which convert chemical stimuli to augmentation of their antigen presentingfunction,虽然他们的反应是different chemicals不需要在phenotypic的统一changes cytokine production or in the induction of apoptosis。

As is well known in the case of Langerhans cells, dendritic cells (DCs) play a crucial role in the initiation of immunity to simple chemicals such as noted in the contact hypersensitivity. Since DCs are scattered in nonlymphoid organs as immature cells. They must be activated to initiate primary antigen specific immune reactions. Therefore, we hypothesized that some simple chemicals must affect the function of DCs. In this paper, we first demonstrated that human monocyte-derived DCs responded to such simple chemicals as DNCB, TNCB, DNFB, NiClィイD22ィエD2, MnClィイD22ィエD2, CoClィイD22ィエD2, SnClィイD22ィエD2, and CdSOィイD24ィエD2 by augmenting their expression of CD86 or HLA-DR antigen, down-regulating c-Fms expression or increasing their production of TNFα. In addition, the DCs stimulated with the chemicals demonstrated increased allogeneic T cell stimulatory function. Next, we found that, among these chemicals, only NiClィイD22ィエD2 and CoClィイD22ィエD2 induced apoptosis in them. Finally, we examined the effects of these chemicals on CD86 expression by 3 different macrophage subsets and DCs induced from the cultures of human peripheral blood monocytes in the presence of M-CSF, M-CSF + IL-4, GM-CSF, and GM-CSF + IL-4, respectively. Among them, only DCs dramatically augmented their expression of CD86. These observations have revealed unique characteristics of DCs which convert chemical stimuli to augmentation of their antigen presenting function, although their responses to different chemicals were not necessarily uniform in the phenotypic changes cytokine production or in the induction of apoptosis.