Cytodifferentiation on cancer of urogenital tract organs via cell to cell interaction

a）前列腺癌细胞（Dunning前列腺腺癌）通过各种胎儿或新生儿间充质的细胞间相互作用的细胞分化将新生大鼠间充质（子宫和阴道）或胎儿大鼠间充质（食管、胃、小肠）或胎儿睾丸雌性化小鼠的泌尿生殖窦间充质（UGM）与R3327 Dunning前列腺腺癌（DT）重组。将所得的组织重组体移植到雄性无胸腺小鼠的肾囊下1个月。在这项检查中，我们可以观察到父母DT的细胞分化，由未分化的单层或多层鳞状或立方上皮细胞内衬的小导管组成。这种组织重组显示了由低柱状上皮细胞内衬的扩大的导管。然而，大鼠UGM和DT的组织重组表现出由高柱状上皮细胞内衬的扩大的导管。这种诱导差异可能是由雄激素的数量引起的 ...更多信息 本研究通过大鼠尿生殖窦间充质细胞（UGM）对人膀胱癌细胞（T-24）的细胞间相互作用诱导分化。将所得到的组织重组体移植到雄性无胸腺小鼠的肾包膜下1 - 2个月。本实验观察了未分化移行细胞癌T-24细胞的分化情况。这种组织重组显示出由低柱状上皮细胞内衬的扩大的导管。前列腺特异性抗原（PSA）局部表达于高柱状上皮细胞。上皮标记尿生殖窦上皮与大鼠UGM结合，证实胎儿间质通过上皮-间质相互作用诱导人膀胱癌细胞分化。少

a) Cytodifferentiation of prostatic cancer cells (Dunning prostatic adenocarcinoma) via cell to cell interaction by various fetal or neonatal mesenchymeThe neonatal rat mesenchymes (uterine and vagina) or fetal rat mesenchymes (esophagus, stomach, small intestine), or urogenital sinus mesenchymes (UGM) of fetal testicular feminization mouse were recombined with R3327 Dunning prostatic adenocarcinoma (DT). The resultant tissue recombinations were grafted under the renal capsule of male athymic mouse for 1 month. In this examination, we could observed the cytodifferentiation of the parental DT composed of small ducts lined by undifferentiated simple or multilayered squamous or cuboidal epithelial cells. Such tissue recombinations showed the enlarged ducts which were lined by low columnar epithelial cells. However, tissue recombination of rat-UGM and DT exhibited the enlarged ducts lined by tall columnar epithelial cells. This inductive difference might be caused by the number of androgen … More receptor in mesenchymal cells.From this result, we could confirm that the mesenchyme produces some cytodifferentiated factors via androgen receptor.Cytodifferentiation of human bladder cancer (T-24) via cell to cell interaction by fetal rat urogenital sinus mesenchyme (rat UGM)Human bladder cancer cells line (T-24) was recombined with rat-UGM. The resultant tissue recombination was grafted under the renal capsule of male athymic mouse for between 1and 2 months. In this examination, we could observed the cytodifferentiation of the T-24 of undifferentiated transitional cell carcinoma cells. Such tissue recombination showed the enlarged ducts which were lined by low columnar epithelial cells. Prostatic specific antigen (PSA) locally expressed on the tall columnar epithelial cells. The epithelial labeling urogenital sinus epithelium combined with rat-UGM.From this result, we could confirm that fetal mesenchyme induce the cytodifferentiation of human bladder cancer cells via epithelial-stromal interaction. Less