Effect of insulin on naturally occurring gingivitis rats with diabetes

糖尿病多发性贫血导致了周期性疾病的衰老，以及糖尿病多发性贫血和周期性疾病之间的关系已被广泛地审查。We used naturally occurring gingivitis rat （ODUS/Odu） to induce diabetes mellitus with an injection of streptozotocin （STZ ：65 mg/kg，i.v.）。STZ注射后四周，我们每天管理胰岛素到第二组（STZ-胰岛素：STZ-入）。STZ注射后8周，固体液体石蜡被注射到边缘。四天后，从3组（STZ，STZ-In， Control ： Cont）收集了周围宏图。使用膜过滤器方法，使用48井微化学箱进行了测量。齐莫桑激活的血清被用作一种刺激的宏观化学物质。Moreover， we measured body weight， blood glucose， plaque index， pocket probing depths （at the center of each mandibular incisor）， triglyceride and hemoglobin A-D21C-D2。在STZ组中，身体重量和血糖水平都很清楚地恢复了。在STZ中的口袋探测深度明显比康特更深。STZ上的宏观化学轴与收缩有显著的减少相比，但STZ和STZ之间没有显著的区别。研究人员建议胰岛素只对口袋探测深度或化学氧烷产生较轻的恢复效果。

Diabetes mellitus causes aggravation of periodontal diseases, and the relationship between diabetes mellitus and periodontal disease has been examined extensively. We used naturally occurring gingivitis rat (ODUS/Odu) to induce diabetes mellitus with an injection of streptozotocin (STZ : 65mg/kg, i.v.). Four weeks after STZ injection, we administered insulin daily to a second group (STZ-Insulin : STZ-In). Eight weeks after STZ injection, sterilized liquid paraffin was injected peritoneally. Four days later, the peritoneal macrophages were collected from the 3 groups (STZ, STZ-In, Control : Cont). The chemotaxis of macrophages was measured by the membrane filter method using a 48-well microchemotaxis chamber. Zymosan activated serum was used as a stimulant of macrophage chemotaxis. Moreover, we measured body weight, blood glucose, plaque index, pocket probing depths (at the center of each mandibular incisor), triglyceride and hemoglobin AィイD21CィエD2. In the STZ-In group, the body weight and blood glucose levels had clearly recovered. Pocket probing depths in STZ was significantly deeper than Cont. Macrophage chemotaxis on STZ was significantly decreased compared to Cont, but there was no significant difference between STZ and STZ-In. The findings suggested that insulin has only a slight recovery effect on pocket probing depth or chemotaxis.